Deproteinisation with acetonitrile (along with methanol or other reagents) is a useful and rapid technique in analysis of drugs or their metabolites in human serum. In this paper application of this simple technique in biopharmaceutical analysis using capillary electrophoresis (CE) is evaluated. Some drugs with different ionic and protein binding properties were selected and dissolved in human ...

Aliquots (25 pl ) were derivatized with phenylisothiocyanatc, vacuum dried and dissolved in 100 pl of 5 mwphosphatc buffer, p H 7.4, containing 5% (v/v) acetonitrile. Ten microlitres was applied to a Pico-Tag column (1 5 cm x 3.9 cm) maintained at 38°C. T h e column eluent was monitored at 254 nm. Two eluents A [70 mM-sodium acetate at pH 6.4 containing 6 % (v/v) acetonitrile] and B [60% (v/v) ...

We describe the emission spectral properties of two rhenium metal±ligand complexes with one and two-photon excitation, Re(bpy)2(CO)3Cl and [Re(bpy)(CO)3CH3CN] ‡, where bpy is 2,20-bipyridyl and CH3CN is acetonitrile. Similar emission spectra and intensity decay times characteristic of the metal-to-ligand charge transfer state were observed for oneand two-photon excitation. The lifetime and quan...

Ion association of the ionic liquid [bmim][Cl] in acetonitrile and in water was studied by dielectric spectroscopy for salt concentrations c ≤ 1.3 M at 298.15 K and by measurement of molar electrical conductivities, Λ, of dilute solutions (c ≤ 0.006 M) in the temperature range 273.15 ≲ T/K ≤ 313.15. Whilst acetonitrile solutions of [bmim][Cl] exhibit moderate ion pairing, with an association co...

A simple and rapid method using HPLC was developed for the determination of tricresyl phosphate (TCP) in polyvinyl chloride (PVC) articles. A test sample was extracted with acetonitrile at 37 degrees C overnight. The extract solution diluted with an equivalent amount of water was applied to a Sep-Pak C18 cartridge, and TCP was eluted with acetonitrile-water (2:1) mixture. The eluate was analyze...

In the present study the solubility of beta-cyclodextrin and 2-hydroxypropyl-beta-cyclodextrin at sub-zero and elevated temperatures (-10 and +30 degrees C) for a given composition of methanol/water and acetonitrile/water binary mixtures (Xs = 0.16) was studied. Moreover, the freezing temperature profiles of acetonitrile-based chromatographic mobile phases were measured, and the obtained result...

The binding interactions of Cu(2+), an essential trace metal ion, and Cd(2+), a deleterious metal ion, with 2-(4'-N,N-dimethylaminophenyl)oxazolo[4,5-b]pyridine (DMAPOP) were studied in acetonitrile and sodium dodecyl sulphate (SDS). The studies show that the surfactant can formulate the metal ions to bind at a specific binding site. In acetonitrile, the Cu(2+) binds at the dimethylamino nitrog...

The work describes the accelerated enzymatic digestion of several proteins in various solvent systems under microwave irradiation. The tryptic fragments of the proteins were analyzed by matrix-assisted laser desorption/ionization mass spectrometry. Under the influence of rapid microwave heating, these enzymatic reactions can proceed in a solvent such as chloroform, which, under traditional dige...

A review about the analyte pKa and buffer pH variations in RP-HPLC mobile phases with the changes in the organic modifier content (acetonitrile or methanol) is presented. A model to accurately predict the pH of particular mobile phases for several commonly used buffers (acetic, citric and phosphoric acid and ammonia systems) in acetonitrile-water and methanol-water mixtures is described. Linear...

Arene ruthenium complexes [(h-arene)Ru(sacc)2(OH2)] (arene 1⁄4 para-cymeme, benzene) containing an aqua and two saccharinato ligands have been synthesized from [(h-arene)RuCl2]2 and sodium saccharinate in a water-ethanol mixture (1:1). The aqua complex [(h-MeC6H4Pr)Ru(sacc)2(OH2)] reacts with acetonitrile to give the acetonitrile complex [(h-MeC6H4Pr)Ru(sacc)2(NCMe)]. The corresponding benzene ...