Potential energy calculations were employed to examine the effect of ribose 2’-O-methylation on the conformation of GpC. Minimum energy conformations and allowed conformational regions were calculated for 2’MeGpC and Gp2’MeC. The two lowest energy conformations of 2’MeGpC and Gp2’MeC are similar to those of GpC itself. The helical RNA conformation (sugar pucker-C(3’)-endo, w’ and w,g-g-, bases-...

Intravenous fluid therapy can alter plasma acid-base balance. The Stewart approach to acid-base balance is uniquely suited to identify and quantify the effects of the cationic and anionic constituents of crystalloid solutions on plasma pH. The plasma strong ion difference (SID) and weak acid concentrations are similar to those of the administered fluid, more so at higher administration rates an...

where Ca and Cb are the analytical (starting) concentration of the acid and base, respectively. Over the past 50 years, chemists have elaborated upon Charlot’s method so that variations upon the “proton accounting” theme are plentiful. The investigators are correct to note that a properly written proton balance equation will not violate the conservation of charge and makes explicit use of charg...

The main objective of this study was to develop an effective potentiometric saturation titration protocol for determining the aqueous intrinsic solubility and the solubility-pH profile of ionizable molecules, with the specific aim of overcoming incomplete dissolution conditions, while attempting to shorten the data collection time. A modern theory of dissolution kinetics (an extension of the No...

Until recently, due to the absence of other suitable approaches, equilibrium concentrations in acid-base systems have been studied exclusively by measuring pH a solution. However, this method can not be used for organic (non-aqueous) solvent solutions. It is known that ionic strength solution, which fundamental component assessing activity coefficient and thermodynamic dissociation constant an ...

BACKGROUND Nitrosative and acid stress play an important role in the pathogenesis of asthma. The aim of this study was to evaluate whether, in asthmatics, a link exists between the concentrations of nitrite/nitrate, ammonia and pH values in exhaled breath condensate (EBC) and asthma severity, lung function, exhaled nitric oxide (F(ENO)), total IgE, eosinophil cationic protein (ECP) and blood eo...

conclusions consequently, small diameter scnt interaction with nucleic acid bases is noncovalent. also, the results revealed that small diameter scnt interaction especially scnt (4, 4) with nucleic acid bases can be useful in practical application area of biomedical fields such detection and drug delivery. objectives hence, the quantum mechanics (qm) method based on ab initio was used for this ...