The structure of proteins plays a crucial role in their functioning. We have shown how SPR technique allows to determine the shape of proteins and its changes under influence of external conditions. Adsorption of matrix M1 protein of influenza virus on self-assembled monolayer of mercaptohexadecanoic acid was studied by SPR. Acid groups on substrate surface provide its negative charge similar t...

Parkinson’s disease is associated with the deposition and accumulation of -synuclein fibrils in the brain. A30P and A53T mutations have been linked to the early-onset familial disease state. Timeresolved tryptophan fluorescence energy-transfer measurements have been used to probe the structures of pseudo-wild-type and mutant (A30P) -synucleins at physiological pH (7.4), in acidic pH (4.4) solut...

In this work, the ability of Na(2)SO(4) and ionic liquids to induce the formation of acidic aqueous two-phase systems (ATPS) is investigated. Ternary phase diagrams, tie lines, and tie-line lengths for several systems were determined and reported at 298 K and atmospheric pressure. It is here shown that among the ionic liquids studied only those containing long alkyl side chains at the ions and/...

The occurrence of 5-hydroxytryptamine (serotonin) in normal human urine is suggested by the experiments of Page and his co-workers. Twarog & Page (1953) examined acetone extracts of urine by a pharmacological method (isolated heart of Venus mercenaria) and reported a 'serotonin-like activity' equivalent to 0'1-10yeg. of 5-hydroxytryptamine/ ml. ofurine. Later, Bumpus&Page (1955) described indol...

Diverse enveloped viruses enter cells by endocytosis and fusion with intracellular compartments. Recent evidence suggests that HIV also infects permissive cell lines by fusing with endosomes in a pH-independent manner. This finding highlights the importance of time-resolved monitoring of viral uptake. In the present study, we designed an imaging-based assay to measure endocytosis in real-time t...

Background The aim of this in vitro study was to evaluate the color stability of CAD/CAM Zirconia ceramics following exposure to acidic drink (Coca Cola) and after exposure to staining solution (coffee). Material and Methods All the samples were immersed in different staining solutions over a 28-day test period. A colorimetric evaluation according to the CIE L*a*b* system was performed by a b...

The objective of this in vitro study was to investigate the effects of viscosity changes of different acidic solutions on dental erosion. Bovine enamel samples (n = 240, Ø = 3 mm) were embedded in acrylic resin and were allocated to 30 groups (n = 8). Citric acid (CA) and phosphoric acid (PA) solutions at pH 2.5, 3, and 3.5 were prepared in de-ionized water (titratable acidity to pH 5.5: 31 ± 0...

Ozone is used in many applications in the industry as an oxidising agent for example for bleaching and sterilisation. The decomposition of ozone in aqueous solutions is complex, and is affected by many properties such as, pH, temperature and substances present in the water. Additives can either accelerate the decomposition rate of ozone or have a stabilising effect of the ozone decay. By contro...

Dissolved aluminum (III) in acidic soils or culture media is often rhizotoxic (inhibitory to root elongation). Alkaline solutions of Al are also sometimes rhizotoxic, and for that reason toxicity has been attributed to the aluminate ion, Al(OH)(4) (-). In the present study, seedlings of wheat (Triticum aestivum L. cv Tyler) and red clover (Trifolium pratense L. cv Kenland) were cultured in aera...

Abstract Spent vanadium catalysts of sulfuric acid production (main elemental composition in wt%: 7.5 V, 9.1 K, 10.2 S, 23.2 Si and 1.4 Fe) can be used as a secondary source vanadium. Extraction was studied using two-step leaching (acidic reductive) spent with further oxidizing solutions. The factors hydrolysis temperature, concentration (H2SO4, Na2SO3) ((NH4)2S2O8) reagents, solid/liquid ratio...