sperm is produced by the testis and mature in the epididymis. for having a successful conception, the fertilizing sperm should have functional competent membranes, intact acrosome, functional mitochondria and an intact haploid genome. the effects of genetic and environmental factors result in sperm vulnerability to damage in the process of spermatogenesis and maturation. in recent years, the fe...

background: oxidative stress in teratozoospermic semen samples caused poor assisted reproductive techniques (art) outcomes. among antioxidants, ascorbic acid is a naturally occurring free radical scavenger and as such its presence assists various other mechanisms in decreasing numerous disruptive free radical processes. objective: the main goal of this study was to evaluate potential protective...

introduction: in the present investigation, we aimed to study the effect of different concentrations of ascorbate/vitamin c, on membrane integrity, viability, and acrosome reaction of human spermatozoa in presence of ferrous (fe2+) ions as lipid peroxidation (lpo) promoter. material and methods: we used 12 semen samples from healthy donors in shahrekord. the samples were then washed twice with ...

The spermatozoa of some species of Diptera belonging to the Psychodidae are described. In the 4 species examined, they appear needle-shaped, non-motile, anteriorly flattened, posteriorly bifid. Flagella, and any microtubule system, are lacking, and the only organelles encountered are an extremely elongated nucleus, a prominent acrosome, a well developed subacrosomal body, a slender, somewhat el...

Background: Low levels of reactive oxygen species (ROS) and calcium are necessary for sperm function. NADPH oxidase 5 (NOX5) is a membrane enzyme which produces ROS. This enzyme is dependent on calcium for its activity. We investigated the importance of NOX5 and an important calcium channel (CatSper) on sperm function.Methods: This laboratory in-vitro study was done in Shiraz, Iran, 2016. Norma...

Spermatozoa sexing requires a diluent that can protect and provide an optimal environment to maintain the quality of spermatozoa. This study targets determine effectiveness adding sucrose tris-egg yolk on sperm swamp buffaloes produced by with egg albumin method. Semen was collected once week for 12 consecutive weeks artificial vagina from three male at Indonesian Center Animal Research Develop...

In the present paper, we introduce a transgenic mouse line whose sperm express green fluorescent protein (GFP) in their acrosome and red fluorescent protein (RFP) in their mitochondria [B6D2F1- Tg(CAG/su9-DsRed2, Acr3-EGFP)RBGS002Osb]. The dual fluorescent sperm showed normal fertilizing ability in both in vivo and in vitro fertilization and the sperm could be observed through uterine and ovidu...

Spermatozoon acrosome reaction is an exocytotic event of the utmost importance for the development of mammalian fertilisation. Current evidence shows that the triggering of the acrosome reaction (AR) could be regulated by the action of diverse compounds, namely, metabolites, neurotransmitters and hormones. The aim of the present review is to describe the modulating effects of several compounds ...

October 2005 | Volume 3 | Issue 10 | e347 | e353 Viewed under a microscope, the coronavirus appears almost beautiful, thanks to the halo-like crown formed by its surface proteins. (“Corona” means “crown” in Latin.) Aesthetics aside, this genus of viruses is responsible for a wide range of animal and human diseases, from the common cold to the deadly severe acute respiratory syndrome, familiarly...