The telomere repeat amplification protocol (TRAP) for the human reverse transcriptase, telomerase, is a PCR-based assay developed two decades ago and is still used for routine determination of telomerase activity. The TRAP assay can only reproducibly detect ∼ 2-fold differences and is only quantitative when compared to internal standards and reference cell lines. The method generally involves l...

(i) For any x1 ∈ R+, there exists some Kx1 and x1 > 0 such that ∂f2 ∂x2 (x1, t) ≤ − x1 for all t ≥ Kx1 . (ii) For any x2 ∈ R+, there exists some Kx2 and x2 > 0 such that ∂f1 ∂x1 (t, x2) ≤ − x2 for all t ≥ Kx2 . In the following result, which is a correction of Theorem 5.1 in [1], f is required to satisfy the same conditions as in Section V of [1] as well as Assumption A above. Theorem 5.1 Assum...

We prove two completeness results, one for the extension of dependence logic by a monotone generalized quantifier Q with weak interpretation, weak in the meaning that the interpretation of Q varies with the structures. The second result considers the extension of dependence logic where Q is interpreted as “there exists uncountable many.” Both of the axiomatizations are shown to be sound and com...

The accurate evaluation of telomerase activity, a typical cancer biomarker, is vital for early screening. In this study, we developed dark-field microscopy (DFM) visual single-particle detection scheme to detect activity based on automatic counting gold nanoparticles (AuNPs). This method started with attaching the substrate (TS) primer magnetic beads (MBs) through streptavidin-biotin interactio...

Many construction projects suffer delays. Their delay risk exacerbates as become larger with more complex schedules, because of the ‘ripple effect’, phenomenon that an individual activity can spread, accumulate, and quantifiably impact project finish. Its magnitude depends on intricate network schedule structure. Yet criticality is currently assessed based static durations. And index merely cou...

Morin is one of the flavonoids with intensive antioxidant activity. With aim to use its benefits on human health, there an increasing trend pay attention content in food or supplements. The simplicity and low cost spectrophotometric determination based formation a morin complex Zn 2+ ion (stoichiometric ratio 1 : 1), at pH 7.98 392 nm, give it advantage over other methods that can be used for q...