The AML1 gene encoding the DNA-binding a-subunit in the Runt domain family of heterodimeric transcription factors has been noted for its frequent involvement in chromosomal translocations associated with leukemia. Using reverse transcriptase-polymerase chain reaction (RT-PCR) combined with nonisotopic RNase cleavage assay (NIRCA), we found point mutations of the AML1 gene in 8 of 160 leukemia p...

The biological roles of the soluble granulocyte colony-stimulating factor (G-CSF) receptor, which arises as a result of alternative RNA splicing, are as yet unknown. In this study, we examined the in vitro effect of a chimeric protein composed of the extracellular region of a murine G-CSF receptor and the human IgG1 Fc region because a human natural soluble G-CSF receptor was not available. Fir...

The effects of media conditioned by leukemic cells from 11 acute myeloblastic leukemia patients on the growth of autologous blast progen itors were studied. First, it was shown that T-cell-depleted leukemic cells from some patients release high levels of colony-stimulating activity into the culture medium, whereas following further depletion of phagocytic cells, the levels of colony-stimulating...

Myeloid sarcoma is a tumor of myeloblast or poorly differentiated myeloid cells that can occur in extramedullary sites, as isolated tumor, concomitantly with or after the diagnosis of acute myeloblastic leukemia. Despite considerable improvement achieved in the outcome of patients with acute myeloblastic leukemia by using intensive chemotherapy and/or hematopoietic stem cell transplantation, it...

The production of colony-stimulating factor (CSF) by the peripheral blood cells of untreated patients with acute myeloid leukemia (AML) was measured in the agar culture system using normal human bone marrow as the source of colony-forming units (CFUc). CSF production was found to be variable and was related to the morphologic subtype of AML--cells from patients with monocytic leukemia produced ...

The AML1 gene encoding the DNA-binding alpha-subunit in the Runt domain family of heterodimeric transcription factors has been noted for its frequent involvement in chromosomal translocations associated with leukemia. Using reverse transcriptase-polymerase chain reaction (RT-PCR) combined with nonisotopic RNase cleavage assay (NIRCA), we found point mutations of the AML1 gene in 8 of 160 leukem...

1. Markovic O, Markovic N: Chediak-Higashi similar granules in acute promyelocytic leukemia. Proceedings of The 9th Congress of Macedonian Physicians 2:1191, 1974 2. Markovic N, Henderson E: Subcellular morphology and prognosis of acute leukemia. Proceedings of The 4th Congress of Yugoslavian Internists 1:375, 1973 3. Akashi K, Shibuya T, Harada M, Oogami A, Teshima T, Takamatsu Y, Kikuchi M, N...

LOOD CELLS are derived from small numbers of selfB renewing pluripotent hematopoietic stem cells that reside in the bone marrow and generate progenitor cells committed to proceed along one of the maturation pathways. Because the life span of blood cells is limited, the production rate of blood cells in the marrow is high, even during steadystate conditions. The marrow system has the ability to ...

A series of monoclonal antibodies have been characterized that define four surface antigens (MY3, MY4, MY7, and MY8) of human myeloid cells. They were derived from a fusion of the NS-1 plasmacytoma cell line with splenocytes from a mouse immunized with human acute myelomonocytic leukemia cells. MY3 and MY4 are expressed by normal monocytes and by greater than 90% of patients with acute monocyti...