We have previously shown the existence of migratory hematopoietic stem cells in adult solid organs. This study demonstrates that granulocyte colony-stimulating factor (G-CSF) and lisofylline, a phosphatidic acid inhibitor that suppresses hematopoiesis-inhibiting cytokines, can enhance the engraftment of organ-based hematopoietic stem cells. When syngeneic heart grafts or liver nonparenchymal ce...

Spontaneous cerebrospinal fluid (CSF) fistula in congenital malformations of the inner ear is rare, and it is found most commonly in infants and children with impaired hearing. Spontaneous CSF otorrhea in adults is often due to defects in the tegmen and the bone overlying the posterior fossa in an otherwise normal ear. In the literature, there are few reports of adultonset spontaneous CSF otorr...

It was shown in children that serum procalcitonin was the best marker to use to differentiate bacterial from viral meningitis. To evaluate procalcitonin in the diagnosis of acute bacterial and viral meningitis, we conducted a prospective study including adult patients who were suspected of having meningitis and who were admitted to an emergency department. Cerebrospinal fluid (CSF) and serum le...

The treatment of adult patients greater than 55 to 70 years of age with acute myelogenous leukemia (AMLI is associated with a treatment-related mortality of approximately 25%. This prospective, double-blind randomized study was designed to see if the use of granulocyte-macrophage colony stimulating factor (GM-CSF; yeast-derived) could shorten the period of neutropenia and to determine any effec...

To examine the influence of the hematopoietic microenvironment on hematopoietic cell proliferation and differentiation during the yolk sac phase of hematopoiesis, we have recently established cell lines from embryonic yolk sac visceral endoderm (YSE) and mesoderm (YSM). In the present experiments, we compared in vitro growth of adult murine bone marrow high proliferative potential colony-formin...

The adult brain functions within a well-controlled stable environment, the properties of which are determined by cellular exchange mechanisms superimposed on the diffusion restraint provided by tight junctions at interfaces between blood, brain and cerebrospinal fluid (CSF). These interfaces are referred to as "the" blood-brain barrier. It is widely believed that in embryos and newborns, this b...

The expression and function of a receptor tyrosine kinase, c-kit, in the adult bone marrow of the mouse were investigated by using monoclonal antibodies (mAbs) against the extracellular domain of murine c-kit. In adult C57BL/6 mouse, 7.8% of total bone marrow cells express c-kit on their surface. Half of the c-kit+ cells do not express lineage markers including Mac-1, Gr-1, TER-119, and B220, w...

Background: Meningitis is a common life threatening infection in neonatal period. Diagnostic value of CSF-CRP level in bacterial meningitis in children and adults has been studied worldwide, but there are limited studies on CSF-CRP in neonatal meningitis. This study conducted to assess any relation between CSF-CRP and plasma CRP levels and abnormal CSF findings in neonates suspected to meningit...

Long-term adult NIH 1 Swiss mouse bone marrow cultures (2.0 X 1 0 cells/ 1 OmI Corning flask. Fisher’s medium. 25 % horse serum. 1 07-M hydrocortisone) were infected with RNA type-C viruses at wk 4. and weekly removed cells were assayed for number. morphology. granulocyte-macrophage progenitor cells (CFUc). pluripotent hemopoietic stem cells (CFUs). and growth in liquid culture in 1 0 % WEHI-3 ...