The major player for catabolite repression is the phosphotransferase systems (PTSs) and cAMP-Crp. Moreover, Cra controls the carbon flow in the metabolic network. In the present research, the effect of modulating cra gene (Δcra) on the consumption of multiple carbon sources such as glucose and fructose (as well as xylose) was investigated under both aerobic and anaerobic conditions. It was show...

Metabolism generates oxygen radicals, which contribute to oncogenic mutations. Activated oncogenes and loss of tumor suppressors in turn alter metabolism and induce aerobic glycolysis. Aerobic glycolysis or the Warburg effect links the high rate of glucose fermentation to cancer. Together with glutamine, glucose via glycolysis provides the carbon skeletons, NADPH, and ATP to build new cancer ce...

Most tumor cells show different metabolic pathways than normal cells. Even under the conditions of sufficient oxygen, they produce energy by a high rate of glycolysis followed by lactic acid fermentation in the cytosol, which is known as aerobic glycolysis or the Warburg effect. Lung cancer is a malignant tumor with one of the highest incidence and mortality rates in the world at present. Howev...

Background: An economical and integrated high-throughput primary screening strategy was developed for high-aerobic microbe Monascus purpureus cultivation. A novel and effective mixture culture method was proposed and used to realize the whole mutant library being high-throughput screened after mutagenesis. Results: The good correlation of fermentation results between differing-scale cultivation...

The enrichment of enhanced biological phosphorus removal (EBPR) sludge with Tetrasphaera was obtained for the first time using glutamate as sole carbon source. Two configurations were tested, an SBR with anaerobic/aerobic sequence and a continuous anaerobic/anoxic/aerobic (A/O) pilot plant. Better results and improved stability was obtained in the continuous A/O operation. Accumulibacter were a...

Much progress has been made since the discovery that some yeasts can ferment D-xylose to ethanol. 1 Initially, only a few yeasts were found that could produce ethanol from D-xylose. Today, at least eight species of yeasts are known to produce significant amounts of ethanol from D-xylose. 2,4-6 Three of these species have been studied extensively: Pachysolen tannophilus, Candida shehatae, and Pi...

HESSELTINE, C. W. (Northern Regional Research Laboratory, Peoria, Ill.), AND K. SHIBASAKI. Miso. III. Pure culture fermentation with Saccharomyces rouxii. Appl. Microbiol. 9:515-518. 1961.-Excellent miso has been prepared with soybean grits inoculated with a pure culture of Saccharomyces rouxii strain NRRL Y-2547. Pure culture inoculum of this osmophilic yeast was prepared by growing the cultur...