This study aimed to prepare the basic components of aflatoxin B1 (AfB1) radioimmunoassay (RIA) technique using liquid phase to measureAfB1 in agricultural commodities. These components were polyclonal antibody (anti-AfB1), I-labelledAfB1 and aflatoxin B1 standards.Anti-AfB1 polyclonal was raised by immunizing six white New-Zealand rabbits with aflatoxin B1bovine serum albumin (AfB1-BSA) conjuga...

Fischer 344 rats readily develop liver cancer when exposed to aflatoxin B1 (AFB1) but dietary administration of the antioxidant ethoxyquin (EQ) provides protection against hepatocarcinogenesis. Chemoprotection by EQ is accompanied by the overexpression of enzymes which detoxify activated AFB1. Aflatoxin-protein adduct formation takes place following metabolism of AFB1 to the dialdehydic form of...

Moldy and split peanut kernels were separated from peanuts exported from Brazil, Sudan, India and Taiwan by visual inspection. The remaining peanuts from Brazil, Sudan and India were roasted lightly and the skins were removed. Stained peanuts were separated from the others. Aflatoxin was detected in moldy and stained peanuts. There was a positive correlation between % of aflatoxin-contaminated ...

the aims of the present study was to evaluate the level of aflatoxin b1 (afb1) in feedstuffs (corn silage, corn, dried alfalfa, cottonseed meal, cottonseed, soybean meal, canola meal, barley, wheat bran, concentrate, sugar beet pulp and wheat straw) that collected from dairy herds in khorasan razavi province that classified according to milk yield in very high (>10000 kg/day), high (5000-10000 ...

Peanut seeds were prepared with variations in roasting conditions. Positive correlations were obtained between loss of aflatoxins in the products and the roasting conditions. Seeds dryroasted at 140C for 40 min resulted in 58.8% and 64.5% reductions in AFB1 and AFG1, those roasted at 150C for 25 minutes resulted in 68.5% and 73.3% reductions in AFB1 and AFG1, respectively. Roasting at150C for 3...

Organically produced spices and herbs were analyzed for determination of aflatoxin B1 (AFB1) by ELISA using immunoaffinity column. For this purpose 93 organic spices and 37 organic herbs were randomly selected from organic markets and organic shops in Turkey. AFB1 was detected in 58 organic spice and 32 organic herb samples. Among organic spice samples, the maximum value was detected in cinnamo...

Tarhana is a popular traditional Turkish cereal-based fermented food product mainly produced at home or at home-scale level. Some certain mould species can grow even at low moisture and pH values and produce aflatoxins in food. This study was conducted to determine aflatoksin levels in tarhana. For this purpose, a total of 138 tarhana powder samples were collected from bazaars in Istanbul and a...

Both aflatoxin and the human immunodeficiency virus (HIV) cause immune suppression and millions of HIV-infected people in developing countries are chronically exposed to aflatoxin in their diets. We investigated the possible interaction of aflatoxin and HIV on immune suppression by comparing immune parameters in 116 HIV positive and 80 aged-matched HIV negative Ghanaians with high (> or =0.91 p...

Title: Association Between Aflatoxin B1 Albumin Adduct Levels and Tuberculosis Infection Among HIV+ Ghanaians. Background: Aflatoxin exposure has been shown to cause cell-mediated immune suppression and enhance HIV viral replication. Such immune suppression from aflatoxin can impair resistance to both infectious diseases and chronic infections. Methods: Hazard ratios (HRs) with 95% confidence i...