The metabolism of aflatoxin B, has been investigated in male rhesus monkeys given i.p. injections of a mixture of ring-labeled aflatoxin B!-'4C and nonradioactive toxin, at a total dose of 0.4 mg/kg. Thirty-five % of the administered radioactivity was excreted in urine within 96 hr. The excretion rate reached a maximum 1 hr after dosing and then fell rapidly, but urine excreted after 96 hr stil...

The in vivo activation of aflatoxin B1 (AFB1) was assessed by using two transgenic mouse lines, M2 and M10, in which the human fetus-specific CYP3A7 was expressed in the kidney (M2) and the liver (M10), respectively. Male mice of 8 weeks old from these two lines were treated with a single i.p. injection of AFB1 (4 mg/kg body weight). AFB1-N7-guanine adduct was quantified by high-performance liq...

Aflatoxin B(1) is a contaminant of agricultural and dairy products that can be related to mutagenic and carcinogenic effects. In this report we explore the capacity of alpha-mannan (Man) to reduce the DNA damage induced by AFB(1) in mouse hepatocytes. For this purpose we applied the comet assay to groups of animals which were first administered Man (100, 400 and 700 mg/kg, respectively) and 20 ...

BACKGROUND Aflatoxins are toxic fungal metabolites enable to contaminate a wide range of natural substrates. This contamination can be host-specific for different plant species. In this study, the ability of a toxigenic Aspergillus parasiticus to produce various aflatoxins on major Iranian cereals was evaluated with special focus on plant susceptibility to toxin production at cultivar level. ...

effect of protexin to diminish negative consequences of feeding aflatoxin b1 (afb1) contaminated diet on performance, immune response, meat quality and ileal microbial flora were examined using 320 seven-day-old japanese quails fed four treatments [control diet (without additives), diet containing 2.5 mg afb1/kg, diet containing 150 mg protexin/kg, diet containing 2.5 mg afb1/kg+150 mg protexin...

The Food and Agriculture Organization of the United Nations estimates that 25% of the food crops in the world are contaminated with aflatoxins. That represents 100 million tons of food being destroyed or diverted to non-human consumption each year. Aflatoxins are powerful carcinogens normally accumulated by the fungi Aspergillus flavus and A. parasiticus in cereals, nuts, root crops and other a...

The exposure to aflatoxin B1 (AFB) in animal-feed processing plants was assessed using binding of AFB to serum albumin. The albumin fraction was digested with pronase, and the digest was purified on a C18 Sepak column and an aflatest affinity column before quantification by ELISA. The level of detectability was 5 pg/mg albumin. The workers served as their own controls, as blood samples were tak...

From measurements of thermal hyperchromicity and the behaviour of an aflatoxin-DNA mixture on a Sephadex coluimn it was concluded that aflatoxin B1 is capable of weak binding to single-stranded DNA. The interactions of the aflatoxins (B1, Gl and G2) with nucleosides result in difference spectra and suggest that the purine bases and the amino group play a role in the binding of all the aflatoxin...

abstract in order to evaluate the effect of testa on reduction of fungal growth and aflatoxin b1 producthon, ten cultivars of pistachio were collected from different parts of pistachio belt i-e rafsanjan, damghan and ghazvin of iran. a section of testa and one mm of endosperm were scraped. 20 gr. of wounded kernels and 20 gr. of unwounded kernels were surface sterilized and then placed in petri...

The in vivo formation of covalent aflatoxin B1 (AFB1)-DNA adducts within the rRNA gene sequences of nuclear DNA has been studied in AFB1-treated rats. Liver nuclear DNA, enriched in ribosomal DNA (rDNA) by one round of cesium salt density gradient centrifugation, was treated under buffered alkaline conditions to convert unstable AFB1-N7-guanine adducts to stable AFB1-formamidopyrimidine derivat...