Experiments were conducted to define the growth requirements of Campylobacter pyloridis, a newly discovered organism associated with gastritis and peptic ulcers. Two clinical isolates were streaked onto various media, and growth was assessed semiquantitatively according to relative colony size and extent of growth through the streak. The growth obtained on fresh chocolate agar, composed of GC a...

MIC of antimicrobial compounds, it is essential to have uniform microbial growth on the agar medium; otherwise, it is difficult to determine a sharply defined area of growth inhibition. The conventional agar gradient-plate method has been found to be unsatisfactory for determining the MIC of chemotherapeutic compounds or antibiotics which inhibit filamentous fungi because the latter frequently ...

Stable isotope ratios of hydrogen and oxygen in microbes have been shown to be functions of the corresponding isotope ratios of the water with which the culture medium was prepared, and thus to contain a potential geographic signal. Water can evaporate from agar (solid) media during culturing, changing its isotope ratios. Here we describe the effect of drying on the isotope ratios of water extr...

Four strains of Campylobacter jejuni and four strains of Campylobacter coli were used to compare the quantitative growth of Campylobacter cells on blood agar base no. 2 (Oxoid), brucella agar (BBL Microbiology Systems and Difco Laboratories), campylobacter agar base (Difco), Columbia blood agar base (Difco and Oxoid), and Mueller-Hinton agar (Difco and Oxoid). Columbia blood agar base and blood...

OBJECTIVES To investigate the reliability of cefoxitin and oxacillin for the detection of mecC-positive Staphylococcus aureus. METHODS The susceptibility to cefoxitin and oxacillin of 62 mecC-positive S. aureus isolates was investigated using broth microdilution, agar dilution, Etest and disc diffusion on different types of media. The data were interpreted for the utility of cefoxitin and oxa...

BACKGROUND Coagulase-negative staphylococci (CNS) are a common cause of nosocomial infections. In recent years, an increase in the incidence of methicillin-resistant coagulase-negative staphylococci (MRCNS) has led to the severity of the disease. OBJECTIVES The aim of this study was to isolate and identify MRCNS strains by oxacillin disk agar diffusion, oxacillin agar screening, and polymeras...

Species identification of coagulase-negative staphylococci often requires the determination of novobiocin susceptibility. Although previous investigators have recommended the use of P agar for this purpose, most clinical laboratories do not routinely utilize this medium. For this reason, studies were performed to compare novobiocin susceptibility results obtained with 11 different species of st...

OBJECTIVES The Oxoid M.I.C.Evaluator (M.I.C.E; Thermo Fisher Scientific) comprises an antibiotic gradient on a plastic support. We compared its performance with Etest--a similar product--using BSAC agar dilution as a reference. METHODS Parallel MIC tests were performed by M.I.C.Evaluator, Etest and agar dilution on Iso-Sensitest agar. In total, 9354 organism/strip combinations were tested by ...