An account of our studies on the metabolic fate of toluene, ethyl-, i8opropyland tert.-butyl-benzenes has been given in earlier papers (Smith, Smithies & Williams, 1954a, b, c; Robinson, Smith & Williams, 1955; Robinson & Williams, 1955). The present paper deals with n-propyland n-butyl-benzenes. These two compounds were previously studied by Thierfelder & Klenk (1924), who showed that npropylb...

Benzene dioxygenase and toluene dioxygenase from Pseudomonas putida have similar catalytic properties, structures, and gene organizations, but they differ in substrate specificity, with toluene dioxygenase having higher activity toward alkylbenzenes. The catalytic iron-sulfur proteins of these enzymes consist of two dissimilar subunits, alpha and beta; the alpha subunit contains a [2Fe-2S] clus...

influence the driving force. Thermodynamic quantities determined from homologous series retention throughout the entire composition range may lead to a better understanding of the driving force and fundamental retention mechanism that results from the inherent differences in chromatographic solvation between acetonitrile and methanol. Tan and co-workers (11) showed that stationary-phase composi...

This article surveys some new organometallic chemistry with the bicyclic aromatic hydrocarbon naphthalene. Aspects to be covered include the synthesis and properties of mononuclear complexes having q2, q 4 or q6-bonded naphthalene ligands. Examples of complexes of the latter type are CpM(q6-C,,H,) (M = Cr, V), which because of the lability of the arene ligands (naphthalene ligand effect) are va...

Transcription of the bphA1A2A3A4C1B genes, which are responsible for the conversion of biphenyl and polychlorinated biphenyl to the meta-cleavage products in Rhodococcus sp. strain RHA1, was examined. The bphA1 promoter (P(bphA1)) was identified and was shown to promote transcription induction by biphenyl and ethylbenzene. An 8.8-kb HindIII fragment that promotes transcription induction of P(bp...

Field metabolomics and laboratory assays were used to assess the in situ anaerobic attenuation of hydrocarbons in a contaminated aquifer underlying a former refinery. Benzene, ethylbenzene, 2-methylnaphthalene, 1,2,4- and 1,3,5-trimethylbenzene were targeted as contaminants of greatest regulatory concern (COC) whose intrinsic remediation has been previously reported. Metabolite profiles associa...

Alkylbenzenes, such as toluene and m-xylene, are an important class of contaminant hydrocarbons that are widespread and tend to accumulate in subsurface anoxic environments. The peripheral pathway for the anaerobic oxidation of toluene in bacteria consists of an initial activation catalyzed by a benzylsuccinate synthase (encoded by bss genes), and a subsequent modified β-oxidation of benzylsucc...