Amperometric biosensors for the measurement of biogenic amines in fish and fruit samples, of lactulose in milk and of glycerol in wine have been developed. The covalent immobilization of enzymes as diamine oxidase (amines), ? galactosidase + fructose dehydrogenase (lactulose) and glycerokinase + glycerol-3P oxidase (glycerol) has been carried out onto polymeric supports for the realization of e...

It has been recognized for some time that biogenic amines occur in a wide range of foods, among them meat and meat products. Meat is an important component of the diet in developed countries. The presence of these amines in food is of interest for two reasons: firstly, for toxicological reasons, in the sense that high levels of dietary biogenic amines can be toxic for certain consumers, and sec...

Cyclic AMP appears to be involved in several excitatory actions of amines on neurones of the Limulus cardiac ganglion. Amines selectively increase levels of cardiac ganglion cyclic AMP with a magnitude and time course similar to that observed for amine-induced excitation of cardiac ganglion burst rate. With respect to either the physiological or biochemical effect, the apparent order of potency...

Polyamines cause inward rectification of Kir K(+) channels by blocking deep within the channel pore. We investigated structural constraints of polyamine block of strongly rectifying mutant K(ATP) channels (Kir6.2[L164C,N160D,C166S] + SUR1). We studied three groups of polyamine analogs: 1) conformationally restricted linear tetra-amines with a cycloalkyl or alkene group between the second and th...

Biogenic amines are biological active compounds that play a significant role in regulation of animal physiology. In insects, the main amines are trace amines like octopamine and tyramine. Those two amines seem to be a partial counterparts of epinephrine and norepinephrine present in vertebrates. Little is known about activity of those compounds in beetles, hence we examined effects of 4 amines ...

A simple, precise, and accurate hydrophilic interaction liquid chromatographic (HILIC) method has been developed for the determination of five aromatic amines in environmental water samples. Chromatography was carried out on a bare silica column, using a mixture of acetonitrile and a buffer of NaH(2)PO(4)-H(3)PO(4) (pH 1.5, containing 10mM NaH(2)PO(4)) (85:15, v/v) as a mobile phase at a flow r...

Formation of histamine, tryptamine, phenylethylamine and tyramine (vasoactive amines) in dry fermented sausage Petrovská klobása during drying and ripening in traditional room (B1) and industrial ripening chamber (B3) were investigated. Dansyl chloride derivatized vasoactive amines were determined using HPLC-DAD on Eclipse XDB-C18 column. Histamine, the most important amine from food safety poi...

Novel tertiary branched tetra-amines, quaternary branched penta-amines, linear penta-amines and linear hexa-amines were distributed as the major polyamines in six obligately extremely thermophilic eubacteria belonging to Thermoleophilum, Bacillus or Hydrogenobacter. The major polyamine of Thermoleophilum album and Thermoleophilum minutum was identified as a quaternary branched penta-amine, 4,4-...

Amines are important for new particle formation and subsequent growth in the atmosphere. Consequently, processes involved receiving more attention recent years. Here, we conduct a field observation order to investigate atmospheric particulate amines at background site Yangtze River Delta (YRD) during summer of 2018. Four PM2.5, i.e., methylamine (MA), dimethylamine (DMA), diethylamine (DEA), tr...

Biogenic amines regulate the proximate mechanisms underlying most behavior, including those that contribute to the overall success of complex societies. For honey bees, one crucial set of behaviors contributing to the welfare of a colony is involved with nest thermoregulation. Worker honeybees cool the colony by performing a fanning behavior, the expression of which is largely influenced by res...