In lactococci, transamination is the first step of the enzymatic conversion of aromatic and branched-chain amino acids to aroma compounds. In previous work we purified and biochemically characterized the major aromatic aminotransferase (AraT) of a Lactococcus lactis subsp. cremoris strain. Here we characterized the corresponding gene and evaluated the role of AraT in the biosynthesis of amino a...

Docetaxel (DOX) is one of the clinically used chemotherapeutic agents for treatment breast cancer, instead its severe side effects. Noscapine, an antitussive opium alkaloid, on other hand, has shown antitumor activity against a number cancers without any significant Since both compounds targeted tubulin, it interesting to see combined effect management cancer. The binding energy -3.49 and -4.18...

Three enzymatic routes toward ?-hydroxy-?-amino acids by tandem aldol addition–transamination one-pot two-step reactions are reported. The approaches feature an enantioselective addition of pyruvate to various nonaromatic aldehydes catalyzed trans-o-hydroxybenzylidene hydratase-aldolase (HBPA) from Pseudomonas putida. This affords chiral 4-hydroxy-2-oxo acids, which were subsequently enantiosel...