We present a novel method for the rapid measurement of pH fluxes at close proximity to the surface of the plasma membrane in mammalian cells using an ion-sensitive field-effect transistor (ISFET). In conjuction with an efficient continuous superfusion system, the ISFET sensor was capable of recording rapid changes in pH at the cells' surface induced by intervals of ammonia loading and unloading...

Myocardial ischemia is characterized by the liberation of adenosine and by complement-mediated inflammation. We have reported that amidated C3, formed when ammonia (NH3) disrupts the thiolester bond of C3, serves as an alternative pathway convertase, generates C5b-9, and stimulates phagocytic oxidative metabolism. We investigated whether the deamination of adenosine by adenosine deaminase in he...

This paper presents a convenient micromethod for the estimation of amino acids, based on the reaction with ninhydrin. The ammonia formed in this reaction is determined, after elimination of the interference from ninhydrin with superoxol, by addition of saturated potassium hydroxide, aeration, and titration with standard acid. The application of the reaction with ninhydrin is one of the most spe...

this study looks at the communication between users concerning health risks, with the aim of exploring their use of fora and assessing whether participants establish a niche with like-minded users during these exchanges. by integrating a corpus linguistic approach with content analysis and multiple studies on computer mediated health discourse, this study analyses the intense attention paid to ...

treatment by HD show a significantly high ammonia concentration in arterial blood, but not in venous blood. Muscle detoxification may contribute to limiting the rise of ammonia in this condition. contribution of various organs to ammonia formation: a review of factors determining the arterial ammonia concentration. of blood ammonia alterations in uraemic patients under hemodialysis and CAPD. Th...

We evaluated the performance of the Du Pont aca ammonia procedure with regard to (a) linearity, (b) precision, (c) interferences, and (d) effect of anticoagulants. Linearity extends to 2,000 mumol/L. The SD of the method was essentially constant (2 to 3 mumol/L) and independent of the NH3 concentration. Hemoglobin, bilirubin, and lipemia do not interfere. Either EDTA or heparin is suitable as a...

The sensitivity improvement of a flow-injection spectrophotometric method for the determination of ammonia was examined based on an indophenol blue coloration reaction with salicylate and hypochlorite in the presence of manganese(II) as a reaction promotion catalyst. The optimal conditions for achieving higher sensitivity of ammonia determination were examined using a three-line flow system. Th...