G udrun D. Trogisch*, H elm ut Köcher**, and W olfram R. Ullrich* * Institut für Botanik, Technische Hochschule, Schnittspahnstraße 3. D-6100 Darmstadt, Bundesrepublik Deutschland ** Hoechst AG, D-6230 Frankfurt 80. Bundesrepublik Deutschland Z. Naturforsch. 44c, 33 — 38 (1989); received July 13/November 11, 1988 Dedicated to Professor Wilhelm Simonis on the occasion o f his 80th birthday Ammon...

Filamentous fungi are important cell factories. In contrast, we do not understand well even basic physiological behavior in these organisms. This includes the widespread phenomenon of organic acid excretion. One strong hurdle to fully exploit the metabolic capacity of these organisms is the enormous, highly environment sensitive phenotypic plasticity. In this work we explored organic acid excre...

A new substrate, ammonium thymolphthalein monophosphate, is proposed for use in alkaline and acid phosphatase determinations. The ammonium salt shares the advantages of the sodium salt, but is a more stable substrate and results in more stable working reagents. The substrate is buffered at ph 10.1 in diethanolamine for determining alkaline phosphatase activity in 5 mm, and at pH 5.9 in citrate ...

137 Biochar (BC) was evaluated for nitrogen (N) and phosphorus (P) removal from aqueous solution to quantify its nutrient pollution mitigation potential in agroecosystems. Sorption isotherms were prepared for solutions of ammonium (NH4 +), nitrate (NO3 -), and phosphate (PO4–P) using BC of corn (Zea mays L.) and oak (Quercus spp.) feedstock, each pyrolyzed at 350 and 550°C highest treatment tem...

The study of inorganic nutrients dynamics in shallow sediments usually focuses on two main pools: porewater (PW) nutrients and exchangeable (EX) ammonium and phosphate. Recently, it has been found that microphytobenthos (MPB) and other microorganisms can accumulate large amounts of nutrients intracellularly (IC), highlighting the biogeochemical importance of this nutrient pool. Storing nutrient...

High concentrations of animal production in the United States have increased the concern about the environmental fate of phosphorus (P) in manures. We conducted a growth chamber study to develop phosphorus source coefficients (PSCs) for manures and fertilizers typically land applied and incorporated into calcareous soils of the western United States as well as to determine the potential plant P...

Phosphoenolpyruvate carboxylase (EC 4.1.1.31) was purified to homogeneity with about 29% recovery from immature pods of chickpea using ammonium sulfate fractionation, DEAE-cellulose chromatography, and gel filtration through Sephadex G-200. The purified enzyme with molecular weight of about 200,000 daltons was a tetramer of four identical subunits and exhibited maximum activity at pH 8.1. Mg(2+...

By WILLIAM KERPPOLA, M.I).* P HOSPHATASE can be demonstrated in tissues by means of reliable histochemical methods. Such a method! w’as described for alkaline phosphatase in 1939 by Gomori,#{176}’ and in the same year by Takamatsu.2 Two years later Gomori#{176} described his method for demonstrating acid phosphatase. The principle on which these methods are based is to incubate slices of tissue...

In experiments carried out in greenhouses, some authors have shown that ammonium sulphate induces greater soil acidity and salinity than other sources of N. Moreover, nitrif ication inhibitors (NI) tend to cause ammonium to accumulate in soil by retarding its oxidation to nitrate. This accumulated ammonium would also have an effect on soil salinity. Consequently, the aim of this paper was to ev...

Ethylene glycol (EG) consumption is commonly employed as an experimental regimen to induce hyperoxaluria in animal models of calcium oxalate nephrolithiasis. This approach has, however, been criticized because EG overdose induces metabolic acidosis in humans. We tested the hypothesis that EG consumption (0.75% in drinking water for 4 wk) induces metabolic acidosis by comparing arterial blood ga...