In order to investigate certain aspects of protein metabolism, it was desired to prepare glycine labeled with radioactive carbon. The method of preparation was chosen with the object of obtaining the highest possible yields, and techniques suitable for handling small amounts of material were used throughout. The reactions employed and the yields obtained were as follows: The synthesis involves ...

We developed an analytical reverse-phase high-performance liquid chromatographic procedure for rapid assessment of the purity of NADH. The method completely separates adenosine monophosphate and adenosine diphosphoribose from NADH. By use of this analytical technique we found that preparative chromatography on DEAE-cellulose gives NADH that is free from adenine nucleotides as well as other impu...

A series of novel hexadentate enterobactin analogues, which contain three catechol chelating moieties attached to different molecular scaffolds with flexible alkyl chain lengths, were prepared. The solution thermodynamic stabilities of the complexes with uranyl, ferric(III), and zinc(II) ions were then investigated. The hexadentate ligands demonstrate effective binding ability to uranyl ion, an...

By controlling the extent of hydrolysis during the self-assembly process of a zwitterionic-based ligand with uranyl cations, we observed a structural evolution from the neutral uranyl-organic framework [(UO2)2(TTTPC)(OH)O(COOH)]·1.5DMF·7H2O (SCU-6) to the first cationic uranyl-organic framework with the formula of [(UO2)(HTTTPC)(OH)]Br·1.5DMF·4H2O (SCU-7). The crystal structures of SCU-6 and SC...

Near real time process monitoring of the uranium content in an aqueous fuel recycling plant is a desired component of an advanced safeguards suite; Ultraviolet– Visible spectroscopy and Time Resolved Laser induced Fluorescence Spectroscopy can contribute to this technology gap. This work presents the observation of the spectroscopic parameters (molar absorptivities, fluorescent response) of the...

The complex formation of uranyl ions (UO(2+)2) with poly(methacrylic acid) (PMAA) was investigated by potentiometric, conductometric, UV-visible, luminescence and FTIR spectroscopic, and thermal analysis methods. The stoichiometric ratio at complexation between polymer and uranyl ions was found to be equal to 2 polymer base units per uranyl ion. FTIR spectra of the PMAA-UO(2+)2 complex confirme...

To improve our understanding of uranium toxicity, the determinants of uranyl affinity in proteins must be better characterized. In this work, we analyzed the contribution of a phosphoryl group on uranium binding affinity in a protein binding site, using the site 1 EF-hand motif of calmodulin. The recombinant domain 1 of calmodulin from A. thaliana was engineered to impair metal binding at site ...

the main goal of this study is to investigate the capability of zeolites a and p synthesized from iranian natural clinoptilolite for uranium uptak. the removal of uranium(vi) from aqueous solution via ion exchange by zeolites in a single component system with various contact times,  temperatures and initial concentrations of uranium(vi) was investigated. the experimental results were fitted to ...

The conversion of steelmaking slag (a waste product of the steelmaking process) to calcium carbonate (CaCO(3)) was tested using hydrochloric acid, ammonium hydroxide and carbon dioxide via a pH-swing process. Batch reactors were used to assess the technical feasibility of calcium carbonate recovery and its use for pre-treatment of acid mine drainage (AMD) from coal mines. The effects of key pro...

The potential of Helicobacter pylori to degrade gastric mucus was examined. Colonies of H pylori cultured from antral mucosal biopsy specimens of patients with non-autoimmune gastritis were washed with sterile saline, passed through a sterilisation filter, and the filtrate examined for urease, protease, and mucolytic activity. The filtrate failed to hydrolyse bovine serum albumin, or to degrade...