A 70 year old lady of recurrent ischaemic stroke on a chronic urinary catheter presented with purple discoloration of her catheter bag. Clinically, she was febrile. The urinary catheter drainage bag (Panel A) was purple stained. Her urine pH was alkaline at 7.5 with leucocytes present. The urine culture grew Klebsiella pneumoniae. She was treated with ampicillin/sulbactam which led to the resol...

An 80-year-old man was admitted to our hospital with a diagnosis of primary hepatic actinomycosis determined based on a percutaneous aspiration biopsy. The abscesses and state of liquefaction were easily and effectively visualized on contrast-enhanced ultrasonography. Ampicillin/sulbactam was administered; however, lesions of hepatic actinomycosis suddenly infiltrated the diaphragm and right th...

A 53-year-old man was admitted to the hospital with a diagnosis of cellulitis and osteomyelitis. Twenty-four days after the initiation of daptomycin and sulbactam/ampicillin, he developed a fever and pulmonary infiltration. Bronchoalveolar lavage revealed a high number of eosinophils, while an intracutaneous test revealed positivity for daptomycin. The patient improved after discontinuing antim...

A 33-year-old previously healthy man injured his gums and subsequently developed dyspnea and fever. A chest X-ray showed nodules and infiltrates in both lungs, and the patient was initially diagnosed with pneumonia and administered meropenem hydrate, although his symptoms did not improve. A blood culture identified Fusobacterium necrophorum, and thrombophlebitis in the internal jugular vein of ...

We present the case of a 60-year-old Caucasian male with history of hepatitis C viral cirrhosis with portosystemic encephalopathy and ascites with evidence of spontaneous bacterial peritonitis (SBP) with absolute neutrophil count (ANC) of 944 cells/µL blood. Despite adequate treatment, the abdominal pain and elevated creatinine continued to persist. Initial ascites fluid cultures returned back ...

An inducible penicillin beta-lactamase was purified from a strain of Alcaligenes faecalis resistant to beta-lactam antibiotics. The purified enzyme preparation gave a single protein band on polyacrylamide gel electrophoresis, and its molecular weight was 29,000 based on sodium dodecyl sulfate-acrylamide gel electrophoresis. Its isoelectric point was 5.9. The enzyme more rapidly hydrolyzed penic...

Mechanism-based inactivators of b-lactamases are used to overcome the resistance of clinical pathogens to b-lactam antibiotics. This strategy can itself be overcome by mutations of the b-lactamase that compromise the effectiveness of their inactivation. We used PCR mutagenesis of the TEM-1 b-lactamase gene and sequenced the genes of 20 mutants that grew in the presence of ampicillin-clavulanate...

Staphylococcus (S.) aureus is deemed as one of the main pathogens in human and animals. S. can produce various toxins that usually implicated food poisoning. could possess mecA gene, which principle cause β-lactam antibiotics resistance, particularly methicillin-resistant (MRSA). Broiler’s meat worthy for humans, but it may expose to contamination with MRSA during poultry processes slaughterhou...