Proportion of HIV transmission events according to time from infection, if the seroconverter is assumed to have only 1 subsequent sex partner (white bars) or if all subsequent sexual contacts are with susceptible individuals (black bars). Assumptions include that the time from infection to death is 10 years and that transmission does not occur ,during the 5 months before death. 6 months to 7.9 ...

In [18] the authors developed a method for computing normal forms of dynamical systems with a coupled cell network structure. We now apply this theory to one-parameter families of homogeneous feed-forward chains with 2-dimensional cells. Our main result is that Hopf bifurcations in such families generically generate branches of periodic solutions with amplitudes growing like ∼ |λ| 1 2 ,∼ |λ| 1 ...

MATERIALS Bacteriophage T4 DNA ligase T vector Target DNA (25 μg/ml), amplified by PCR When the PCR mixture contains more than one or two bands of amplified DNA, purify the target fragment by electrophoresis through low melting/gelling temperature agarose (please see Recovery of DNA from Low-meltingtemperature Agarose Gels: Organic Extraction). If not purified by gel electrophoresis, PCR-amplif...

Amplified spontaneous emission is usually treated as an incoherent noise process. Recent theoretical and experimental work using rephasing optical pulses has shown that rephased amplified spontaneous emission (RASE) is a potential source of wide bandwidth time-delayed entanglement. Due to poor echo efficiency the plain RASE protocol doesn’t in theory achieve perfect entanglement. Experiments do...

ABBREVIATIONS: AFLP (Amplified Fragment Length Polymorphism); BAC (Bacterial Artificial Chromosome); CAPS (Cleaved Amplified Polymorphic Sequence); CPCSD (California Planting Cotton Seed Distributors); EST (Expressed Sequence Tag); FOV (Fusarium oxysporum f. sp. vasinfectum); FW (Fusarium Wilt); QTL (Quantitative Trait Locus); RAPD (Random Amplified Polymorphic DNA); RIL (Recombinant Inbred Lin...

The genetic status of cyclin genes was examined in a panel of 47 colorectal carcinoma cell lines. Cyclin D2 was found to be amplified in one tumor and cyclin E in another. In each of the two cases, the amplified cyclin gene was overexpressed at the protein or mRNA level. Cyclin Dl, previously shown to be amplified in breast and other tumors, was not amplified in these cancers. These data sugges...

Two methods are described for the detection of in vitro enzymatically amplified DNA. The first method involves solution hybridization of labeled oligonucleotides to amplified products. Hybridization of primers to a dilution series of known concentration of amplified DNA showed that approximately 5 pg of DNA could be detected by this method. In the second method, radiolabeled deoxynucleotides we...

Retrotransposons are abundant in the genomes of plants. In the present study, inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) markers were developed for the cassava genome (Manihot esculenta Crantz). Four cassava cultivars (Fécula Branca, IPR-União, Olho Junto, and Tamboara, two samples per cultivar) were used to obtain IRAP ...

BACKGROUND Neuroblastoma is the most common solid tumour of infancy and is responsible for 15% of childhood cancer deaths. Presence of amplified MYCN in neuroblastoma is associated with poor prognosis and rapid tumour progression. The aim of this study was to examine and compare the ultrastructural features of high-risk MYCN amplified neuroblastomas, with lower-risk non-MYCN amplified tumours. ...

Among the core features of ASD, altered sensitivities in all modalities have been accorded increasing importance. Heightened sensitivity to pain and unusual expressions of and reaction to pain have not hitherto been widely recognised as a presenting feature of ASD in general paediatrics. Failure to recognise ASD as a common cause of pain can lead to late diagnosis, inappropriate treatment, dist...