Selenomonas ruminantium was found to possess two pathways for NH4+ assimilation that resulted in net glutamate synthesis. One pathway fixed NH4+ through the action of an NADPH-linked glutamate dehydrogenase (GDH). Maximal GDH activity required KCl (about 0.48 M), but a variety of monovalent salts could replace KCl. Complete substrate saturation of the enzyme by NH4+ did not occur, and apparent ...

Sporulating clostridial suspensions tend to recycle through subsequent phases such as germination, outgrowth, and further multiplication. The result is a mixture of vegetative cells and of spores at various developmental stages. Recently, activated-culture techniques have been used to achieve almost complete sporulation in semisynchronized populations of several clostridia (Collier, 1). 10, in ...

The BBL Crystal Anaerobe (ANR) identification system was evaluated, and the results were compared with those from conventional anaerobic methods. We tested 322 clinically significant anaerobic bacteria according to the manufacturer's instructions. The system identified correctly 286 of 322 (88.8%) of the anaerobic bacteria tested. Of these, 263 of 322 (81.7%) were identified correctly on initia...

The sequence of changes during germination of putrefactive anaerobe 3679h spores was studied under aerobic conditions in a solution containing l-alanine and sodium pyrophosphate. Evidence that specific changes occurred in two distinct regions of the spore is given by data on several criteria that were used to measure germination. During the initial stage of germination, the absorbancy decreased...

An obligately anaerobic, moderately thermophilic, glutamate-degrading bacterium (strain ZT) was isolated from an enrichment culture obtained from anaerobic thermophilic granular sludge. The cells were rod-shaped to filamentous and showed no motility or spore formation. The cell wall had a Gram-positive structure, which was revealed by electron microscopy. Optimum growth of the strain was observ...

Peptoids (oligo N-substituted glycines) are peptide analogues, which can be designed to mimic host antimicrobial peptides, with the advantage that they resistant proteolytic degradation. Few studies on efficacy of peptoids have focused Gram negative anaerobic microbes associated clinical infections, commonly recalcitrant antibiotic treatment. We therefore studied cytotoxicity and antibiofilm ac...