introduction: development of effective immunodiagnostic methods for detection of mycobacterium tuberculosis infection is crucial. serodiagnostic methods, based on antibody response to specific antigens could provide promising approaches for rapid, economical and easy to perform diagnostic tests which are crucial for tuberculosis (tb) control. in this study, the level of igg antibody responses a...

OBJECTIVE To investigate the clinical significance of anti-nucleosome antibodies in SLE patients lacking anti-double stranded DNA (dsDNA) antibodies. METHODS IgG anti-nucleosome antibodies were detected by enzyme-linked immunosorbent assays (ELISA) in the sera of SLE patients. Anti-dsDNA antibodies were measured by Farr assays and ELISA, not only in the samples taken for anti-nucleosome testi...

Background: Group O blood donor is more readily available and frequently used as a universal red cell emergency whole (EM O) for exchange transfusion in neonates. Sera from group donors contains two separable haemolysin antibodies, anti-A anti-B, cross-reacting antibody called anti-A, B (mostly IgG). The presence of haemolysins the may lead to haemolysis recipients. Objectives: This study aims ...

reactivity. As anti-mitochondria1 antibodies can bind complement we used a complement-fixation test. Results from preliminary tests were difficult to interpret. The Paracoccus membranes had strong anti-complementary activity, which had to be diluted out. Also the sera available from patients with primary biliary cirrhosis were themselves anti-complementary up to quite high dilutions. Neverthele...

Currently marketed Streptococcus pneumoniae vaccines are based on polysaccharide capsular antigens from the most common strains. Pneumococcal histidine triad protein D (PhtD) is a conserved surface protein that is being evaluated as a candidate for a vaccine with improved serotype coverage. Here, we measured the functional activity of human anti-PhtD antibodies in a passive protection model whe...

The presence of anti-alpha-fodrin autoantibodies has been reported to be a highly specific and sensitive test for the diagnosis of Sjögren's syndrome (SjS). We looked (in Nijmegen) for anti-alpha-fodrin, anti-Ro60, and anti-La autoantibodies in a cohort of 51 patients with rheumatic diseases (primary SjS [21], secondary SjS 6, rheumatoid arthritis [RA] 12, systemic lupus erythematosus [SLE] 6, ...

background: toxoplasma gondii is an intracellular protozoan parasite that infects human and animals. toxoplasma parasites are isolated from different parts of ani-mals even from semen but there are little information about the effect of toxo-plasmosis on fertility in animals and humans. in present study, the effect of chronic toxoplasmosis on serum levels of testosterone in men was studied. met...

The routine pretransfusion red blood cell antibody detection test (PADT), performed at the authors' institution, consists of a threephase, saline-antiglobulin technique (immediate spin, 30-minute incubation at 37 °C, IgG indirect antihuman globulin test |IAT|), and each phase is examined for hemolysis and agglutination. To determine if it would be safe to omit reading the 37 °C phase of the PAD...

We have demonstrated that anti-Thy 1.1 and certain of H-2 alloantisera contain an antinuclear factor (ANF) detected by fluorescent antimouse IgG. The incidence of ANF positive antisera that we report may actually be an underestimate. ANF is present in sera not only from our laboratory but also from other sources; thus ANF is not an artifact of our laboratory. ANF is not identical to alloantibod...

objectives the aim of this was to indentify the presence of a/h9n2 virus among different high risk occupational groups, in tehran and qazvin provinces in seasonal outbreak in iran. background in the last decade h9n2 avian influenza viruses had caused outbreaks in poultry in many parts of the world. this subtype could infect other animals such as human and pig. avian h9n2 virus has acquired rece...