β-Glucosidase is an important enzyme for production of ethanol from lignocellulose. With hydrolytic activity on cellooligosaccharides, especially cellobiose, β-glucosidase removes product inhibitory effect on cellulases and forms fermentable sugars. In this study, β-glucosidase encoding gene (BGL1) from traditional starter yeast Saccharomycosis fibuligera BMQ908 was cloned and expressed in Pich...

Diabetes mellitus (DM) is recognized as a serious global health problem that is characterized by high blood sugar levels. Type 2 DM is more common in diabetic populations. In this type of DM, inhibition of α-glucosidase is a useful treatment to delay the absorption of glucose after meals. As a megabiodiversity country, Indonesia still has a lot of potential unexploited forests to be developed a...

Glucosidase II, an asparagine-linked oligosaccharide processing enzyme, is a resident glycoprotein of the endoplasmic reticulum. In kidney tubular cells, in contrast to previous findings on hepatocytes, we found by light and electron microscopy immunoreactivity for glucosidase II predominantly in post-Golgi apparatus structures. The majority of immunolabel was in endocytotic structures beneath ...

Background: p-propoxybenzoic acid (p-PBA) is reported as an active chemical constituent of medicinal plants that possess anti-diabetic activity. It termed a Multiple-Designed Ligand (MDL) having the ability to block more than one enzyme. A molecular docking study justifies binding p-PBA with acarbose and NaVO4 which were considered standard compounds target enzymes. α-amylase inhibition assay w...

The Lauraceae genus Litsea includes many tree species with pharmacological capabilities, including anti-diabetic benefits. Five plant were tested for their activity on α-amylase and α-glucosidase. This study evaluated phytochemical composition, phenolic flavonoid levels, α-glucosidase inhibitor of elliptica, ferruginea, firma, garciae, sp. Phytochemical screening was performed using specific re...

The goal of the current study was to identify potential bioactive components that may be responsible for Rhynchosia beddomei's antidiabetic activity through in silico docking and investigate mechanisms by which beddomei helpful managing diabetes its associated complications. In vitro anti-diabetic assays showed this plant's high efficiency inhibit α-amylase (62.13%) α-glucosidase (59.9%) enzyma...

Isatin, also known as 1H-Indole-2, 3-Dione, is an eight-carbon containing endogenous compound found in nature. Erdmann and Laurent synthesized isatin 1840, before it was discovered It versatile distributed tissues body fluids. Isatins have been detected mammalian tissue, their function a biochemical process modulator has the focus of many debates. The moiety shows some important chemical reacti...

The anti-diabetic potential of whole unripe jackfruit (peel with pulp, flake, and seed) was investigated using inhibitory assays for α-glucosidase, α-amylase, aldose reductase, glycation at multiple stages. Using activity-guided repeated fractionation on a silica gel column chromatography, dietary flavonoid rutin potent antihyperglycemic activity extracted from the methanol extract flour (MJ). ...

Mycobacterium fortuitum and Mycobacterium chelonei are distinguished unambiguously by the combined use of five test characters: nitrate reductase, beta-glucosidase, acid production from fructose, penicillinase, and trehalase. Typically, M. fortuitum was nitrate reductase positive, beta-glucosidase positive; M. chelonei was nitrate reductase negative, beta-glucosidase negative, penicillinase pos...

background: diabetes mellitus is a chronic disease. decreasing postprandial hyperglycemia by retarding glucose absorption through inhibiting carbohydrates digesting enzymes (α-amylase and α-glucosidase) is one of many approaches used for the management of this disease. this study was aimed at evaluating the normoglycaemic potential of helianthus annuus leaf. methods: the effect of the in vitro ...