نتایج جستجو برای: antigen capture elisa

تعداد نتایج: 355188  

Journal: :Advances in Animal and Veterinary Sciences 2015

Bahram Kazemi, Mojgan Bandehpour Negar Seyed Parviz Pakzad Zarrin Sharifnia

Background: Although a simple and direct method does not exist for the detection of chlamydial infections, there are situations in which reliable serological tests, with sensi-tivity related to a specific antigen, could be helpful. Objective: The aim of this study was to clone the first 1100 bp of the C. trachomatis outer membrane protein 2 (omp2) gene in order to prepare a recombinant protein ...

2014
Ge Nie Ting Wang Shengjun Lu Wenqi Liu Yonglong Li Jiahui Lei

BACKGROUND Clonorchiasis, caused by Clonorchis sinensis, is widely distributed in Southeast Asia including China. Clonorchiasis is included in control programs of neglected tropical diseases by World Health Organization (WHO) because it is one of the major health problems in most endemic areas. Diagnosis of clonorchiasis plays a key role in the control programs. However, so far, there is no sat...

Journal: :Journal of clinical microbiology 1979
S A Harding W M Scheld M D McGowan M A Sande

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Streptococcus pneumoniae polysaccharide antigen in cerebrospinal fluid and serum. Sensitivity and specificity were determined for purified antigen preparations. Specificity was also evaluated in the rabbit meningitis model, and the sensitivity was compared to counterimmunoelectrophoresis, using the infected rabbits'...

Abdol Aziz Khezri Ali Ariafar Mehdi Shirazi, Mehrdad Askarian Mehrzad Lotfi Mohammad Amin Afrasiabi Seyyed Mohammad Taghi Ayatollahi

Background: It is relevant to highlight that there is not a precise and perfect report on either 95 percentile value (upper limit of normal range) or on appropriate reference intervals for serum PSA in Iranian population. Objective: To determine age-specific reference ranges for serum prostate-specific antigen (PSA) concentration and PSA density (PSAD) and prostate volumes in a population of he...

2014
Ali ROSTAMI Hossien KESHAVARZ Saeedeh SHOJAEE Mehdi MOHEBALI Ahmad Reza MEAMAR

BACKGROUND Toxoplasma gondii, the obligate intracellular parasite is life threatening in AIDS patients. Diagnosis of toxoplasmosis is based on serological methods especially increasing of IgM and IgG titers, but finding of parasite or its components (antigenemia) may be beneficial method in order to detection of acute toxoplasmosis in immunocompromised patients. METHODS Ninety-four serum samp...

Journal: :Biological & Pharmaceutical Bulletin 2021

Antigen tests for infectious diseases are inexpensive and easy-to-use, but the limit of detection (LOD) is generally higher than that PCR tests, which considered gold standard. In present study, we combined a sandwich enzyme-linked immunosorbent assay (ELISA) with thionicotinamide-adenine dinucleotide (thio-NAD) cycling to improve LOD antigen coronavirus disease 2019 (COVID-19). For recombinant...

2016
Jian Liu Xiangzhong Ye Jizong Jia Rui Zhu Lina Wang Chunye Chen Lianwei Yang Yongmei Wang Wei Wang Jianghui Ye Yimin Li Hua Zhu Qinjian Zhao Tong Cheng Ningshao Xia

Varicella-zoster virus (VZV) is a highly contagious agent of varicella and herpes zoster. Varicella can be lethal to immunocompromised patients, babies, HIV patients and other adults with impaired immunity. Serological evaluation of immunity to VZV will help determine which individuals are susceptible and evaluate vaccine effectiveness. A collection of 110 monoclonal antibodies (mAbs) were obta...

Journal: :Revue scientifique et technique 1993
E M Waithanji V M Nantulya S M Mbiuki

Parasitological diagnosis of Trypanosoma evansi infection in camels is hampered by the small number of parasites in blood circulation, coupled with the tendency of this trypanosome to invade tissues. To overcome this, a more sensitive assay, an antigen enzyme-linked immunosorbent assay (ELISA), was developed, capable of detecting trypanosome antigens released into the bloodstream by dying paras...

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