Sheep fitted with rumen and re-entrant duodenal cannulas were given diets of approximately 200 g hay and 400 g concentrate mixture alone, or supplemented daily with 40 g linseed or coconut oils free or protected with formaldehyde-casein in a 5 x 5 Latin-square arrangement. Chromic oxide paper was given as a marker at feeding time and passage to the duodenum of neutral-detergent fibre (NDF) and ...

In this paper, the composition and biological activities of polysaccharides from tea seed (TSPS) obtained by water extraction were investigated. The properties and chemical compositions of TSPS were analyzed with HPGPC, IC, and IR methods. The results showed that TSPS consisted of three kinds of polysaccharides with the molecular weight of 500 kDa, 130 kDa, and 5 kDa. TSPS consisted of rhamnose...

Coutilization of hexoses and pentoses derived from lignocellulose is an attractive trait in microorganisms considered for consolidated biomass processing to biofuels. This issue was examined for the H(2)-producing, extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus growing on individual monosaccharides (arabinose, fructose, galactose, glucose, mannose, and xylose), mixtures o...

Lenzites betulinus, known as gilled polypore belongs to Basidiomycota was isolated from fruiting body on broadleaf dead trees. It was found that the mycelia of white rot fungus Lenzites betulinus IUM 5468 produced ethanol from various sugars, including glucose, mannose, galactose, and cellobiose with a yield of 0.38, 0.26, 0.07, and 0.26 g of ethanol per gram of sugar consumed, respectively. Th...

Five strains of the yeast Phaffia rhodozyma, NRRL Y-17268, NRRL Y-17270, ATCC 96594 (CBS 6938), ATCC 24202 (UCD 67-210), and ATCC 74219 (UBV-AX2) were tested for astaxanthin production using the major sugars derived from corn fiber. The sugars tested included glucose, xylose, and arabinose. All five strains were able to utilize the three sugars for astaxanthin production. Among them, ATCC 74219...

The E-arabinose operon is under both positive and negative control by the product of its regulatory gene, UrQc. We have measured transcription of the arabinose genes by hybridization of 3H-labeled messenger RNA to single-stranded DNA from an arabinose transducing phage in order to study the effect of the regulatory gene on transcription. In strains in which the arabinose regulatory gene is comp...

The E-arabinose operon is under both positive and negative control by the product of its regulatory gene, UrQc. We have measured transcription of the arabinose genes by hybridization of 3H-labeled messenger RNA to single-stranded DNA from an arabinose transducing phage in order to study the effect of the regulatory gene on transcription. In strains in which the arabinose regulatory gene is comp...

Sugar cane bagasse hemicellulosic fraction was hydrolysed by treatment with 70 mg of sulphuric acid per gram of dry mass at 125 °C for 2 h. The hydrolysate was used as the substrate to grow Candida langeronii RLJ Y-019 at 42 °C; initial pH 6.0; stirring at 700 rev/min and aeration at 1.0 and 2.0 v/v/min. The utilization of D-xylose, L-arabinose, and acetic acid were delayed due to the presence ...

Chinese medicine residues contain abundant cellulose and hemicellulose, which are potential renewable carbon sources for ethanol production. The aim of the present study was to develop a rapid and reliable method to evaluate the cellulose and hemicellulose utilization in Chinese medicine residues. In the present study, key hydrolysates (arabinose, galactose, glucose, xylose, and cellobiose) of ...

BACKGROUND The role of lectins is important in interaction between pathogens and mosquito vectors. This study was performed to identify agglutinin activities of protein molecules on the midgut of Culex pipiens. METHODS Culex pipiens was reared in insectray condition and the midguts of males and females (blood fed and unfed) were dissected separately in Tris-HCl buffer. The extracts of midguts...