Growing evidence suggests that microRNAs (miRNAs) are aberrantly expressed in many human cancers, and that they play significant roles in carcinogenesis and cancer progression. The identification of tumor suppressive miRNAs and their target genes could provide new insights into the mechanism of carcinogenesis. However, the genetic or epigenetic regulations of these miRNAs have not yet been full...

As a complement to the Bovine HapMap Consortium project, we initiated a systematic study of the copy numbervariation (CNV) within the same cattle population using array comparative genomic hybridization (array CGH). Oligonucleotide CGH arrays were designed and fabricated to cover all chromosomes with an average interval of 6 kb using the latest bovine genome assembly. In the initial screening, ...

To identify genetic changes involved in the progression of breast carcinoma, we did cDNA array comparative genomic hybridization (CGH) on a panel of breast tumors, including 10 ductal carcinoma in situ (DCIS), 18 invasive breast carcinomas, and two lymph node metastases. We identified 49 minimal commonly amplified regions (MCRs) that included known (1q, 8q24, 11q13, 17q21-q23, and 20q13) and se...

OBJECTIVE Prader-Willi Sydrome (PWS) is a human disorder related to genomic imprinting defect on 15q11-13. It is characterized by a series of classic features such as hypotonia, hyperphagia, obesity, osteoporosis, typical facial and body dysmorphosis, hypogonadism, mental and behaviour disorders. Our study was designed to precisely detect the microdeletions, which accounts for 65%-70% of the PW...

MOTIVATION Array Comparative Genomic Hybridization (CGH) can reveal chromosomal aberrations in the genomic DNA. These amplifications and deletions at the DNA level are important in the pathogenesis of cancer and other diseases. While a large number of approaches have been proposed for analyzing the large array CGH datasets, the relative merits of these methods in practice are not clear. RESUL...

Disseminated peritoneal leiomyomatosis (DPL) is a rare disease entity belonging to the category of smooth muscle tumours of uncertain growth. It is characterized by proliferation of multiple smooth muscle nodules in the peritoneal cavity mimicking a malignant process such as peritoneal carcinomatosis but which when studied histologically proves to be of benign nature. Its origin is still unknow...

Sequencing. Genomic DNA was extracted from peripheral blood using standard procedures and its quality was monitored using a Nanodrop spectrophotometer. DNA was amplified for CDKN2A, CDK4, MC1R, TP53 and MITF as described previously [13, S1]. PCR products were subjected to automatic sequencing by ABI PRISM 310 genetic analyzer (Applied Biosystems). Microsatellite analysis. The primers used for m...