The formation of cholesterol synthesis inhibiting molecules by five different strains of the koji mold Aspergillus oryzae was studied. After growing these strains on a complex liquid medium we found in crude organic phase extracts and specific fractions there from compounds inhibiting cholesterol synthesis in human hepatic T9A4 cells in vitro at enzyme sites downstream of dihydrolanosterol. Thi...

Gallic acid esters could be synthesized biologically by tannase in organic media, among which is propyl gallate, an antioxidant widely used as a food additive. Efficient intracellular expression of Aspergillus oryzae tannase was achieved in Pichia pastoris under the control of the AOX1 promoter, and the productivity of recombinant tannase was 960 U/L or 64 U/g dry mass. The recombinant P. pasto...

Cr(III), Co(II), Ni(II), Cu(II) and Zn(II) salts of 2-(4-nitrophenylaminocarbonyl)benzoic acid were characterized by physical, analytical and spectroscopic studies and checked for their in-vitro antimicrobial activity against three bacterial strains, Mycobacterium smegmatis (Gram +ve), Escherichia coli (Gram -ve), Pseudomonas aeuroginosa (Gram -ve) and three fungal strains, Nigrospora oryzae, A...

Fungi from the genus Aspergillus are important saprophytes and opportunistic human fungal pathogens that contribute in these and other diverse ways to human well-being. Part of their impact on human well-being stems from the production of small molecular weight secondary metabolites, which may contribute to the ability of these fungi to cause invasive fungal infections and allergic diseases. In...

The generation of royalties on the use foreign-made inoculum strains or starters as a result implementation ‘Nagoya Protocol’ has led to efforts and healthy competition secure useful biological resources in each country. In this study, we recognized necessity importance securing Korea produced using five fungi (Aspergillus oryzae Aspergillus niger). Specifically, quality characteristics exhibit...

Abstract Geodorum densiflorum is an ornamental and medicinally important orchid. The medicinal metabolites are produced by endophytic fungi associated with orchid tissue, particularly flowers. However, there no report of the from G. This research aimed to study different parts flower. Fungal isolation was carried out sepal, petal, labium, stigma, anther using surface sterilization method. were ...

BACKGROUND Breakthrough zygomycosis is increasingly observed among patients at high risk for fungal infection who are receiving voriconazole, reflecting either selective pressure or voriconazole-associated alterations in Zygomycetes virulence. We tested the latter hypothesis, using 2 phylogenetically disparate zygomycosis models. METHODS Three Zygomycetes strains were exposed to voriconazole ...

Marker rescue is an important molecular technique that enables sequential gene deletions. The Cre-loxP recombination system has been used for marker gene rescue in various organisms, including aspergilli. However, this system requires many time-consuming steps, including construction of a Cre expression plasmid, introduction of the plasmid, and Cre expression in the transformant. To circumvent ...

Aspergillus oryzae is generally recognized as safe, but it is closely related to A. flavus in morphology and genetic characteristics. In this study, we tested the aflatoxigenicity and genetic analysis of nine commercial A. oryzae strains that were used in Korean soybean fermented products. Cultural and HPLC analyses showed that none of the commercial strains produced detectable amount of aflato...