Lyme borreliosis (LB) is a common infection of domestic dogs in areas where there is enzootic transmission of the agent Borrelia burgdorferi. While immunoassays based on individual subunits have mostly supplanted the use of whole-cell preparations for canine serology, only a limited number of informative antigens have been identified. To more broadly characterize the antibody responses to B. bu...

The Bordetella pertussis BrkA protein protects against the bactericidal activity of complement and antibody; however, some individuals mount an immune response that overcomes this bacterial defense. To further characterize this process, the bactericidal activities of sera from 13 adults with different modes of exposure to B. pertussis (infected as adults, occupational exposure, immunized with a...

Purified blood group A and B substances have not been known to give precipitin reactions with human sera containing homologous isoagglutinins although they are capable of combining with antibody as evidenced by their capacity to neutralize or inhibit isohemagglutination (1-3). The A and B substances have, however, been found to be antigenic in man (4) and have been used to produce sera of high ...

TITl)\ (JI’ ‘FIlE antigemiic structure of leukocytes amid detectiomi of “leukoagglut inimis’ ‘ have comicermied invest igat ors in immumiology amid hematology for many years. 1 ecemitly, reports of “leukoagglut.inins” iii various pathologic comiditions have appeared in rapidly increasimig miumbers, as reviewed by Dausset..’ Various serologic techmiics have been emiiployed, both in the detectiom...

The objective of the present study was to investigate immunoglobulin G (IgG) and IgA antibody immune responses to Porphyromonas gingivalis, Prevotella intermedia, Bacteroides forsythus, and Candida albicans in the sera of patients with rheumatoid arthritis (RA), the synovial fluid (SF) of patients with RA (RA-SF samples), and the SF of patients without RA (non-RA-SF samples). An enzyme-linked i...

background and aims: the aim of this study was application of gamma irradiated fmdv antigen for liquid phase blocking sandwich elisa (b-elisa) to quantify guinea pig anti fmdv type a87/irn antibody. methods: fmdv type a87/irn was propagated to a titer of 107.5/ml tcid50 and then irradiated by gamma irradiator. the irradiated 146 s antigen was purified by sucrose gradient centrifugation after in...