The present study was conducted to isolate phosphate solubilizing bacteria (PSB) from rhizospheric saline soils of coastal Odisha, India and evaluated their ability. Total four PSB were isolated based on the halo zone formation (solubilizing index 2.63-3.14) PVK agar medium characterized biochemical molecular characteristics as Bacillus subtilis (B1), B. megaterium (B2), Sphingomonas paucimobil...

Advancement in green chemistry has increased the use of microbial hydrolyases various industries and chemical processes because high catalytic efficiency, specificity, cost-effectiveness eco-friendly nature. Bioconversion tannins such as tannic acid is achieved by tannin acyl hydrolase, also known tannase. It converts into glucose gallic catalyzing hydrolysis ester depside linkages acid. Tyrosi...

We characterized catabolite repression of the genes encoding xylose utilization in Bacillus megaterium. A transcriptional fusion ofhy4 encoding xylose isomerase to the spoVG-lacZ indicator gene on a plasmid with a temperature-sensitive origin of replication was constructed and efficiently used for single-copy replacement cloning in the B. megaterium chromosome starting from a single transforman...

Germination of dormant spores of Bacillus species is initiated when nutrient germinants bind to germinant receptors in spores' inner membrane and this interaction triggers the release of dipicolinic acid and cations from the spore core and their replacement by water. Bacillus subtilis spores contain three functional germinant receptors encoded by the gerA, gerB, and gerK operons. The GerA germi...

Nitrogen (N) fixation through legume-Rhizobium symbiosis is important for enhancing agricultural productivity and is therefore of great economic interest. Growing evidence indicates that other soil beneficial bacteria can positively affect symbiotic performance of rhizobia. Nodule endophytic plant growth promoting rhizobacteria (PGPR) were isolated from common bean nodules from Nakuru County in...

A simple two-step procedure for purification of enolase from germinated spores or vegetative cells of Bacillus megaterium is described. The procedure resulted in a 1,200-fold purification with production of homogeneous enzyme in approximately 75% yield; the enzymes from spores and cells seemed identical. The molecular weight of the native enzyme was 335,000, with a subunit molecular weight of 4...

The asymmetry of the DNA duplex due to polynucleotide strand complementarity could be the molecular basis of cell polarity in spore-forming bacteria. To test this possibility, the relationship of DNA strand segregation to the spore location pattern in chains of sporangia was investigated in Bacillus megaterium. Spores containing one chromosomes labelled in one of the complementary strands were ...

AIMS The effect of spore density on the germination (time-to-germination, percent germination) of Bacillus megaterium spores on tryptic soy agar was determined using direct microscopic observation. METHODS AND RESULTS Inoculum size varied from approximately 10(3) to 10(8) cfu ml(-1) in a medium where pH=7 and the sodium chloride concentration was 0.5% w/v. Inoculum size was measured by global...