The present work reports successful DNA amplification of Pantoea agglomerans and Bacillus pumilus through Random Amplified Polymorphic DNA (RAPD). For this, template DNA was obtained without conventional DNA extraction. The procedure was as follows: cultures grown for 20 hours in 5 mL LB medium were centrifuged and the resulting preparation was suspended in TE buffer. After boiling, the cell su...

The radiation response, quantified by the dose-log survival curve, of spores Bacillus pumilus subjected to irradiation in a 150 kV x-ray beam was examined and compared with response obtained reference cobalt-60 beam. were irradiated at doses ranging from 2 kGy 11 kGy. same for both qualities within measurement uncertainties. Combining these findings our previously published data, resistance B. ...

The diversity of thermophilic bacteria was investigated in four hot springs, three salt marshes and 12 desert sites in Morocco. Two hundred and forty (240) thermophilic bacteria were recovered, identified and characterized. All isolates were Gram positive, rod-shaped, spore forming and halotolerant. Based on BOXA1R-PCR and 16S rRNA gene sequencing, the recovered isolates were dominated by the g...

Nine strains belonging to Bacillus cereus group has been isolated from food and environmental samples. Their taxonomic position was confirmed by RFLP analysis of 16S rRNA gene digested with TaqI. The detection of DNA sequences encoding the hemolysin BL complex and enterotoxin NHE, was studied in Bacillus sp. isolates. Set of primers was used to amplify fragment of hblD gene by PCR. For the dete...

A new lipolytic strain of Bacillus sp. was isolated from oil contaminated soil sample. On measurement of biochemical parameters and 16S rDNA sequence information the strain was identified as Bacillus pumilus RK31 (NCBI accession no GQ463238). The lipase purification steps involved, 60% ammonium sulphate saturation, gel filtration chromatography using Sephadex G200 and ion exchange chromatograph...

The objective of this study was to evaluate the antibacterial activity of various extracts of whole plant of Borreria hispida (Linn). The extract was prepared from the whole plant of Borreria hispida by hot continuous percolation method in Soxhlet apparatus with various solvents (Pet. Ether, ethyl acetate, Methanol). All the extracts of Borreria hispida were tested for antibacterial efficacy ag...

Bacterial weathering plays a significant role in rock weathering, yet only limited number of studies were conducted on this topic. The recent rapid developments geomicrobiology are expected to increase the pace research area. extent and duration biological processes mineral samples minerals preferred by bacteria still not fully understood due wide variety both bacterial species types. Biologica...

In the present study, rarely explored spore former (Bacillus safensis & Bacillus pumilus) and non-spore (Arthrobacter luteolus, Chryseomicrobium imtechense Corynebacterium efficiens) alkaliphilic calcifying microbes were evaluated for their prolonged survival in cementitious environment. Extensive experimental program was designed to examine mechanical, self-healing, microstructural modificatio...

Fusarium solani is the causative organism of Fusarium wilt of tomato (Lycopersicon esculentum Mill). Four Bacillus spp identified as B. amyloliquefaciens, B. cereus, B. pumilus and B. subtilis were tested for biocontrol activities in vitro as zone of inhibition and in vivo as percent disease control and disease incidence. The result from in vitro analysis showed that B. amyloliquefaciens inhibi...