background: bacillus subtilis refers to stretched and sometimes curved, gram-positive, aerobic, and catalase-positive bacilli, which has thermo-resistant endospores. it has been known as a normal flora in the human but can be pathogens in the case of opportunistic. also, it can be the pathogen of nosocomial infections such as wound among hospitalized patients. purpose of this study was to ident...

This study investigates the production of crude Ca-independent and low pH active α-amylase by Bacillussp. KR-8104 in submerged fermentation (SmF) and solid-state fermentation (SSF) systems. Differentparameters were evaluated in each system using “one factor at a time” approach to improve the production ofenzyme. The results showed that in the SmF the maximum enzyme production ...

the rose sawfly, arge rosae linnaeus, is one of the most destructive pests of rose bushes in the north of iran. nowadays, many attempts have been made to reduce pesticide application by looking for new methods of pest control. a non chemical method for controlling insect pests including a. rosae can be achieved by using genetically engineered plants expressing carbohydrase inhibitors. therefore...

Di CARLO, F., AND REDFERN, S. 1947 Alpha-amylase from Bacillus subtilis. I. Purification and physical properties. Arch. Biochem., 15, 333-342. DINGLE, T., REID, W. W., AND SOLOMONS, G. L. 1953 The enzymatic degradation of pectin and other polysaccharides. II. Application of the "cup-plate" assay to the estimation of enzymes. J. Sci. Food Agr., 3, 149-155. GOODMAN, T. T. 1950 Adaptive production...

Fermented culture of Streptomyces fulvissimus was found to secrete an antibacterial protein inhibitory to Micrococcus luteus, Bacillus subtilis, Bacillus cereus and methicillin resistant Staphylococcus aureus (MRSA) strains. The extracellular protein from the fermented culture on concentration revealed a high molecular weight peptide of 63kDa on SDS-PAGE gel and the region on gel displayed inhi...

BACKGROUND Secretory expression of valuable proteins by B. subtilis and its related species has attracted intensive work over the past three decades. Although very high yields can be achieved with homologous proteins, production of heterologous proteins by B. subtilis is unfortunately not the straight forward. The Sec pathway is the major route for protein secretion in B. subtilis. Therefore, t...

In our previous study, we compared the two α-amylase enzymes from Bacillus sp.KR8104, BKA∆(N44) and BKA∆(N44C193) which is the secreted form of it. The results indicated that the presence of 193 amino acids propeptide in the C-terminal of BKA∆(N44) changed its enzymatic parameters like an uncompetitive inhibitor in comparison to BKA∆(N44C193). In the present study, we cloned the DNA sequence of...