نتایج جستجو برای: baculovirus expression system

تعداد نتایج: 2988560  

2013
Quan Nguyen Lars K. Nielsen Steven Reid

Baculovirus-insect cell technologies are applied in the production of complex proteins, veterinary and human vaccines, gene delivery vectors' and biopesticides. Better understanding of how baculoviruses and insect cells interact would facilitate baculovirus-based production. While complete genomic sequences are available for over 58 baculovirus species, little insect genomic information is know...

Journal: :Journal of virological methods 2007
Olga A Lihoradova Irina D Ogay Abdusattor A Abdukarimov Sh S Azimova Dwight E Lynn Jeffrey M Slack

An in vitro baculovirus cloning system has been developed for direct cloning of foreign DNA into baculovirus genomes. This system is called the "Homingbac system" because it uses homing endonucleases. The Homingbac system was engineered into the baculoviruses AcMNPV, BmNPV, PxMNPV, RoMNPV, HaSNPV and HzSNPV. All Homingbac viruses were designed to retain the polyhedra phenotype so that they coul...

Journal: :Journal of invertebrate pathology 2011
Manon M J Cox Yoshifumi Hashimoto

The viral surface protein hemagglutinin (HA) has been recognized as a key antigen in the host response to influenza virus in both natural infection and vaccination because neutralizing antibodies directed against HA can mitigate or prevent infection. The baculovirus-insect cell system can be used for the production of recombinant HA molecules and is suitable for influenza vaccine production whe...

Journal: :Journal of virology 2001
R V Merrihew W C Clay J P Condreay S M Witherspoon W S Dallas T A Kost

Our group and others have recently demonstrated the ability of recombinant baculoviruses to transduce mammalian cells at high frequency. To further characterize the use of baculovirus as a mammalian gene delivery system, we examined the status of transduced DNA stably maintained in Chinese hamster ovary (CHO) cells. Four independent clones carrying two introduced markers, the genes for neomycin...

Journal: :The Journal of general virology 2003
Sun U Song Seok-Hwan Shin Soon-Ki Kim Gwang-Seong Choi Woo-Chul Kim Moon-Hee Lee Sei-Joong Kim In-Ho Kim Mi-Sook Choi Young-Jin Hong Kwan-Hee Lee

Efficient gene delivery of a baculovirus-derived vector (BV-p53-lacZ) to a human osteogenic sarcoma cell line, Saos-2, was serendipitously found while evaluating the vector for gene delivery to human p53-null tumour cells in a previous study. Therefore, we investigated other human, rat and mouse osteogenic sarcoma and other types of tumour cell lines for transduction efficiency via baculovirus ...

2011
Xiao Shen Patrik O Michel Qiuling Xie David L Hacker Florian M Wurm

Background Sf-9 cells, derived from Spodoptera frugiperda, are widely used for recombinant protein production using the baculovirus expression vector system (BEVS). However, this results in a productive viral infection and cell lysis. Therefore, a non-lytic, plasmid-based expression system for suspension Sf-9 cells would be a valuable alternative to the BEVS for rapid, scalable, and highyieldin...

Journal: :Folia biologica 2012
A Yousefi F Fotouhi S Hosseinzadeh M T Kheiri B Farahmand S Montazeri F Mousavi

The global outbreak of novel A/H1N1 spread in human population worldwide has revealed an emergency need for producing a vaccine against this virus. Current influenza vaccines encounter problems with safety issues and weak response in high-risk population. It has been established that haemagglutinin is the most important viral antigen ...

2015
Lei Wang ChienWei Chiang Hong Liang Hengyi Wu Weixing Feng Sara K. Quinney Jin Li Lang Li

The use of in vitro metabolism data to predict human clearance has become more significant in the current prediction of large scale drug clearance for all the drugs. The relevant information (in vitro metabolism data and in vivo human clearance values) of thirty-five drugs that satisfied the entry criteria of probe drugs was collated from the literature. Then the performance of different in vit...

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