Introduction. The program SEQIN-ST helps entering and analysing nucleic acid sequences and protein sequences using personal computers of the ATARI ST series. It allows the manual entry and/or the direct entry from a sequencing gel using the graphic mouse. The user decides the entry mode and the layout of the sequence. In check mode the sequence can be entered a second time and is automatically ...

Detecting high-z GRBs is important for constraining the GRB formation rate, and tracing the history of re-ionization and metallicity of the universe. Based on the current sample of GRBs detected by Swift with known redshifts, we investigated the relationship between red-shift, and spectral and temporal characteristics, using the BAT event-by-event data. We found red-shift trends for the peak-fl...

Batch codes, introduced by Ishai, Kushilevitz, Ostrovsky and Sahai, represent the distributed storage of an n-element data set on m servers in such a way that any batch of k data items can be retrieved by reading at most one (or more generally, t) items from each server, while keeping the total storage over m servers equal to N . This paper considers a class of batch codes (for t = 1), called c...

A number of manifold learning algorithms have been recently proposed, including locally linear embedding (LLE). These algorithms not only merely reduce data dimensionality, but also attempt to discover a true low dimensional structure of the data. The common feature of the most of these algorithms is that they operate in a batch or offline mode. Hence, when new data arrive, one needs to rerun t...

The influence of aeration and glucose feeding on the stability of recombinant protein A in Escherichia coli during the transition period from a fed-batch cultivation to downstream processing was studied. Neither interruption of the feeding under aerobic conditions nor anaerobic conditions in presence of glucose could stabilize protein A completely and the intracellular ATP pool did not decrease...

Industrial fermentations conducted in a batch or semi-batch mode demonstrate significant batch-tobatch variability. Current batch process monitoring strategies involve manual interpretation of highly informative but low frequency offline measurements such as concentrations of products, biomass and substrates. Fermentors are also fitted with computer interfaced instrumentation, enabling high fre...

Although serum-free media have been widely used in mammalian cell culture for therapeutic protein production, the effects of serum-substitutes on product quality have not been extensively examined. This study observed an adverse effect of Primatone RL, an animal tissue hydrolysate commonly used as a serum-substitute to promote cell growth, on sialylation of interferon-gamma (IFN-gamma) derived ...

Vero cells were grown adherent to microcarriers (Cytodex 1; 3 g L(-1)) using animal component free media in stirred-tank type bioreactors. Different strategies for media refreshment, daily media replacement (semi-batch), continuous media replacement (perfusion) and recirculation of media, were compared with batch cultivation. Cell densities increased using a feed strategy from 1×10(6) cells mL(...

Batch distillation is an important separation process for small-scale production especially in pharmaceutical, specialty chemical and biochemical industries. Although batch distillation units require lower capital cost than continuous units, the unsteady state nature of the process, results in higher operating costs. Optimal control in batch distillation is a mode of operation which allows us t...

A mathematical model for fed-batch culture of hybridoma cells [24] has been employed for generating simulation data in this study. The model is a seventhorder nonlinear model where both glucose and glutamine concentrations are used to describe the specific growth rate, μ. The cell death rate, kd, is governed by lactate, ammonia and glutamine concentrations. The specific MAb production rate, qMA...