Sympathetic neuronal death induced by nerve growth factor (NGF) deprivation requires the macromolecular synthesis-dependent translocation of BAX from the cytosol to mitochondria and its subsequent integration into the mitochondrial outer membrane, followed by BAX-mediated cytochrome c (cyt c) release. The gene products triggering this process remain unknown. Here, we report that BIM, a member o...

objective(s): juglone is isolated from many species of the juglandaceae family and used as an anti-viral, anti-bacterial, and anti-tumor therapeutic. here, we evaluated juglone-induced antitumor effect in ovarian cancer skov3 cells. materials and methods: mtt assay was performed to examine juglone anti-proliferative effect. cell cycle and apoptosis were studied using flow cytometry in juglone-tr...

The "BH3-only" proapoptotic BCL-2 family members initiate the intrinsic apoptotic pathway. A small interfering RNA knockdown of BIM confirms this BH3-only member is important for the cytokine-mediated homeostasis of hematopoietic cells. We show here that the phosphorylation status of BIM controls its proapoptotic activity. IL-3, a hematopoietic survival factor, induces extracellular signal-regu...

Bax is a proapoptotic protein implicated in cell death involved in several neurodegenerative diseases. Intracellularly expressed antibody (Ab) fragments (intrabodies) inhibiting Bax function would have potential for developing therapeutics for the aforementioned diseases and can serve as research tools. We report identification, cloning, and functional characterization of several Bax-specific s...

The transcriptional regulator Yin Yang-1 (YY1) is a tumor suppressor known to be overexpressed in pancreatic cancer. We found that overexpression of YY1 promoted apoptosis and increased the expression and mitochondrial localization of the pro-apoptotic Bax protein in pancreatic cancer cell lines. Luciferase reporter, electrophoretic mobility shift (EMSA), and chromatin immunoprecipitation (ChIP...

The aim of our study was to evaluate the immunohistochemical expression of Bcl-2 and Bax protein in optic nerve axons after severe eyeball injury and in the eyes with absolute glaucoma. A series of 19 eyeballs, enucleated because of absolute glaucoma and 41 eyeballs, enucleated, following extensive injury, at the Department of Ophthalmology of the Medical University of Bialystok, were taken int...

Loss of the pro-apoptotic Bcl-2 family protein Bax occurs in ~50% of hereditary nonpolyposis colorectal cancer (HNPCC) due to microsatellite instability (MSI). Recently, we found that some of the "Bax-negative" MSI tumor cells contain a functional Bax isoform, BaxΔ2, which sensitizes cells to selective chemotherapeutics. Here we show the detection of Bax microsatellite mutations and expression ...

Cell apoptosis induced by UV irradiation is a highly complex process in which different molecular signaling pathways are involved. PUMA has been proposed as an important regulator in UV irradiation-induced apoptosis. However, the molecular mechanism through which PUMA regulates apoptosis, especially how PUMA activates Bax in response to UV irradiation is still controversial. In this study, base...

Background: Rosmarinic acid (RA) is a natural phenolic compound that acts as Fyn inhibitor by 53 homology modeling of the human structure. Therefore, apoptosis mechanism related to NF-κB signaling pathway induced RA in HepG2 was investigated. Methods: The cell growth, apoptosis, and proliferation regulated various concentrations were studied. proteins expression MMP-2, MMP-9, PI3K, AKT, NF-κB, ...

Background and objectives: Epidemiological studies have shown that high dose of ethanol can lead to apoptosis. On the other hand, the consumption of medicinal plants such as turmeric in the diet and performing physical activities are considered as factors to control apoptosis. Therefore, this article aims to mainly evaluate the anti-apoptotic effects of swimming and curcumin interactively durin...