Targeted delivery of IFNgamma to tumors has been achieved by fusing this cytokine with GCNGRC, a tumor neovasculature homing peptide. Although the therapeutic efficacy of this protein (called IFNgamma-NGR) in animal models is greater than that of IFNgamma, frequent administrations of IFNgamma-NGR may result in lower efficacy and tumor resistance. We investigated the role of indoleamine 2,3-diox...

In the spindles of HSC (High Speed Cutting) machines with rolling bearings, higher temperatures in bearings can be expected, which may affect resistance to movement bearing itself. Therefore, estimate these resistances, it is necessary know components. The article presents results FEM simulation tests temperature distribution a bearing. These studies were focused on assessing influence such fea...

Comprehensive geotechnical modelling of the behaviour of piles generally encounters setbacks: in preparing model bed simulating field conditions, of specific and uniform shear strength, and reproduction for repetitions. Due to such limitations in traditional practices, information on the complex mechanism of shaft-base resistance interaction is not adequately known. Other outcomes are: varied r...

Influence of different types surfactants on electrodeposition copper- and carbon-bearing (graphite, carbon nanotubes (CNTs)) composite powder has been experimentally investigated. The size particles decreased, corrosion resistance increased when were added. Addition cationic surfactant CTAB to the electrolyte with simultaneous ultrasonic treatment for CNTs dispersion gives maximum effect.

There is an urgent need for more durable and cost-effective bearing and gear steels for wind turbine applications; yet we are still using traditional steels such as 8620 and 52100 steels, which are inexpensive and have high strength. However, higher corrosion resistance is required for marine application of wind turbines. Our research group developed under NSF funding a series of high strength ...

The gene for leader peptidase, termed lep, was mapped to the region between purI and nadB at min 54 to 55 on the Escherichia coli chromosome. Mapping involved (i) cloning the gene into the plasmid pBR322, (ii) transforming the plasmid into a polA strain where it cannot replicate autonomously, (iii) selecting by ampicillin resistance the rare cell in which the plasmid had recombined into the chr...

INTRODUCTION Selection of the K103N mutation is associated with moderately reduced in vitro fitness of HIV. Strains bearing K103N in vivo tend to persist, even in the absence of additional drug pressure, as minority quasispecies, often undetectable in genotyping resistance testing assays, performed at standard conditions. Here, we report on the rapid and long lasting selection of a K103N bearin...

In planta transformation methods are now commonly used to transform Arabidopsis thaliana by Agrobacterium tumefaciens. The origin of transformants obtained by these methods has been studied by inoculating different floral stages and examining gametophytic expression of an introduced beta-glucuronidase marker gene encoding GUS. We observed that transformation can still occur after treating flowe...

Rates of infection with antibiotic-resistant bacteria have increased precipitously over the past several decades, with far-reaching healthcare and societal costs. Recent evidence has established a link between antibiotic resistance genes in human pathogens and those found in non-pathogenic, commensal, and environmental organisms, prompting deeper investigation of natural and human-associated re...

Symmetric, dimeric daclatasvir (BMS-790052) is the clinical lead for a class of picomolar inhibitors of HCV replication. While specific, resistance-bearing mutations at positions 31 and 93 of domain I strongly suggest the viral NS5A as target, structural mechanism(s) for the drugs' activities and resistance remains unclear. Several previous models suggested symmetric binding modes relative to t...