The responses of sea cucumbers to salinity changes are reflected in the expression profiles multiple genes and microRNAs. miR-2008 Pleckstrin Homology Domain Containing A3 (PLEKHA3) were previously shown be involved cucumbers. To better understand relationship between PLEKHA3, PLEKHA3 predicted interaction was investigated using luciferase reporter assays gene knockdowns. Dual-luciferase analys...

Purpose: To investigate the expression of miR-125b and vitamin D receptor (VDR) in renal cell carcinoma (RCC) assess biological function RCC. Methods: We used quantitative real-time polymerase chain reaction (RT-PCR) to detect nucleic acids western blotting analyze protein abundance RCC lines. MiR-125b mimic inhibitor were employed behavior The relationship between miR-125 VDR was verified usin...

Luciferase-based bioluminescence is a technique used for non-invasive analysis of molecular functions in living cells. New recombinant luciferase and advanced CCD (Charge Coupled Device) cameras allow the detection of bioluminescent signals at submicron resolution. These recombinant luciferase proteins have the potential to facilitate great advances in the study of membrane dynamics at both the...

Enzymes are deformable molecules: the molecular structure can exist in essentially a continuum of different conformations. These are accessible by applying mechanical stress to the molecule. Mechanics then provides a general molecular mechanism to modulate enzymatic reactions, by applying mechanical stresses which deform the enzyme. There are approximately 10 known different enzymatic reactions...

Genetically encoded bioluminescent indicators for intracellular Ca2+ are described here with CaM-M13 interaction-induced complementation of split Renilla luciferase. The Ca2+-induced interaction between CaM and M13 leads to complementation of the N- and C-terminal halves of split Renilla luciferase in living cells. This intramolecular interaction results in the spontaneous and simultaneous emis...