Simian Virus 40 (SV40) is a non-enveloped virus which has been shown to pass from surface caveolae to the endoplasmic reticulum (ER) in an apparently novel infectious entry pathway. We now show that the initial entry step is blocked by brefeldin A (BFA) and by incubation at 20°C. Subsequent to the entry step, the virus reaches a domain of the rough ER by an unknown pathway. This intracellular t...

Here, we give a complete description of the entire c-Boomerang Connectivity Table for Gold function over finite fields even characteristic, by using double Weil sums. As by-product, generalize result Boura and Canteaut (IACR Trans. Symmetric Cryptol. 2018(3) : 290–310, 2018) classical boomerang uniformity (see also extended abstract Eddahmani Mesnager at Boolean Functions their Applications (BF...

We have used a 58 kDa membrane protein (p58) as a marker to study the transport pathway between the rough endoplasmic reticulum (ER) and the Golgi apparatus. Immunolocalization of p58 in fibroblasts showed its presence in a single cisterna and in small tubular and vesicular elements at the cis side of the Golgi apparatus. In addition, the protein was detected in large (200-500 nm in diameter) t...

Caveolins are a crucial component of caveolae but have also been localized to the Golgi complex, and, under some experimental conditions, to lipid bodies (LBs). The physiological relevance and dynamics of LB association remain unclear. We now show that endogenous caveolin-1 and caveolin-2 redistribute to LBs in lipid loaded A431 and FRT cells. Association with LBs is regulated and reversible; r...

The major milk proteins, the caseins, contain multiple phosphorylation sites. Phosphorylation of the caseins is necessary to allow Ca2+ binding and aggregation of the caseins to form micelles. We have followed the phosphorylation of the caseins in isolated acini from lactating mouse mammary gland. Incubation of mammary cells with [32P]orthophosphate revealed that phosphorylation of newly synthe...

The fungal metabolite brefeldin A (BFA) induces the disassembly of the Golgi complex in mammalian cells. The drug seems to accentuate tubule formation and causes the subsequent fusion with the endoplasmic reticulum (ER). To investigate the biochemical requirements and kinetics of BFA-induced Golgi disassembly, we have reconstituted the process of green fluorescent protein-tagged Golgi complex d...

Apraxia is a disorder in executing “learned”1 or “skilled” movement (excluding explicitly symbolic movements2) not accounted for by weakness, incoordination, sensory loss, incomprehension or inattention to commands.3, 4 In a classic case, the patient carried out commands such as, “show me how you would use a hammer” with his right arm without hesitation.1 However, when asked to perform the same...

ADP-ribosylation factors (ARF) are small G proteins that play key roles in vesicular transport processes. We have studied the distribution of ARF1 in live cells using chimeras of ARF1 mutants (wild type (wt) ARF1; Q71L-ARF1 (reduced GTPase); T31N (low affinity for GTP); and (Delta)Nwt (deletion of amino acids 2-18)) with green fluorescent protein (GFP). Confocal microscopy studies showed that t...

This paper investigates the relay beamforming design for quality of service (QoS) discrimination in two-way relay networks. The purpose is to keep legitimate two-way relay users exchange their information via a helping multiantenna relay with QoS guarantee while avoiding the exchanged information overhearing by unauthorized receiver. To this end, we propose a physical layer method, where the re...