Glial cell-line derived neurotrophic factor (GDNF) is a secreted protein with great therapeutic potential. However, in order to analyse the interactions between GDNF and its receptors, researchers have been mostly dependent of radioactive binding assays. We developed a FACS-based binding assay for GDNF as an alternative to current methods. We demonstrated that the FACS-based assay using TGW cel...

Radioligand binding assays take advantage of the ability to detect the decay of radioactive material, which can be incorporated into a ligand of choice. The interaction of such a radioligand with a receptor preparation can subsequently be determined by capturing and measuring the amount of radioactivity present. Radioligand binding assays can be used to estimate molecular parameters, such as th...

DNA nanotechnology has conquered the challenge of positioning quantum emitters in hotspot optical antenna structures for fluorescence enhancement. Therefore, origami serves as scaffold to arrange nanoparticles and emitters, such fluorescent dyes. For next optimizing applicability plasmonic hotspots molecular assays, a Trident structure that increases accessibility is introduced, thereby improvi...

The HIV protease inhibitor nelfinavir is currently being analyzed for repurposing as an anticancer drug many different cancers because it exerts manifold off-target protein interactions, finally resulting in cancer cell death. Xenosensing pregnane X receptor (PXR), which also participates the control of proliferation and apoptosis, was previously shown to be activated by nelfinavir; however, ex...

We compared the analytical and clinical performance of two free-thyroxine (FT4) assays--a solid-phase radioimmunoassay, Spectria, and a time-resolved fluoroimmunoassay, Delfia, both of them two-step methods--with the performance of a direct radioimmunoassay, Nichols, to measure FT4 concentration in equilibrium dialysate of undiluted serum. The three assays showed comparable analytical performan...

Coordination in honeybees is heavily dependent on their chemoreception system, which odorant-binding proteins are very important. A previous study based the antennal transcriptome of Apis cerana revealed that OBP15 was significantly up-regulated at 25-day-old. To further unravel and clarify its functional role, we first cloned characterized AcerOBP15 from antennae worker bees. Sequence phylogen...

•ATO rescues multiple p53 mutants effectively in various assays •The structural mechanism of how mutant function is restored by ATO described •Most are stabilized structurally but only some transcriptionally rescued •Widely applicable, yet has individual mutation-based therapeutic potential TP53 the most frequently mutated gene cancer, these mutations remain therapeutically non-actionable. Majo...

Influenza virus PA endonuclease has recently emerged as an attractive target for the development of novel antiviral therapeutics. This is an enzyme with divalent metal ion(s) (Mg(2+) or Mn(2+)) in its catalytic site: chelation of these metal cofactors is an attractive strategy to inhibit enzymatic activity. Here we report the activity of a series of N-acylhydrazones in an enzymatic assay with P...

Accurate measurement of sex hormone binding globulin (SHBG) by 5 alpha-dihydrotestosterone (DHT) binding assays requires the establishment of acceptable upper and lower limits of labelled DHT binding and the use of optimal quantities of radioinert DHT to displace labelled DHT. A computer assisted binding assay for SHBG is described in which upper and lower binding limits are derived mathematica...

The dermorphin-derived peptide [Dmt1]DALDA (H-Dmt-d-Arg-Phe-Lys-NH2; Dmt, 2',6'-dimethyltyrosine) labels mu-opioid receptors with high affinity and selectivity in receptor binding assays. In previous studies, [Dmt1]DALDA displayed a mechanism of action distinct from that of morphine, as evidenced by its insensitivity to antisense probes reducing morphine analgesia and incomplete cross tolerance...