Clathrin-mediated endocytosis (CME) is the major pathway of epidermal growth factor receptor (EGFR) internalization. It is commonly believed that CME mediates long-term attenuation of EGFR signaling by targeting the receptor for degradation. However, the EGFR can also be internalized through (a) clathrin-independent pathway(s), and it remains unclear why distinct mechanisms of internalization h...

Proteolytic machines powered by ATP hydrolysis bind proteins with specific peptide tags, denature these substrates, and translocate them into a sequestered compartment for degradation. To determine how ATP is used during individual reaction steps, we assayed ClpXP degradation of ssrA-tagged titin variants with different stabilities in native and denatured forms. The rate of ATP turnover was 4-f...

Pollution of the oceans by microplastics (<5 mm) represents a major environmental problem. To date, a limited number of studies have investigated the level of contamination of marine organisms collected in situ. For extraction and characterization of microplastics in biological samples, the crucial step is the identification of solvent(s) or chemical(s) that efficiently dissolve organic matter ...

............................................................................................. vi JUSTIFICATION ........................................................................................ 1 METHODS ................................................................................................ 2 ..................................................................... RESULTS AND SIGNIF...

Purpose To characterize the clinical and biological properties of biodegradable collagen matrices (BCMs) for possible glaucoma drainage device implantation. Methods A total of 68 refractory glaucoma eyes, followed up postoperatively for at least 6 months, were consecutively enrolled after retrospective chart review. The BCM-augmented Ahmed valve implantations (BAAVI) using our Ologen-6 and Ol...

Multiprotein complexes in the cell are dynamic entities that are constantly undergoing changes in subunit composition and conformation to carry out their functions. The protein-DNA complex that promotes recombination of the bacteriophage Mu is a prime example of a complex that must undergo specific changes to carry out its function. The Clp/Hsp100 family of AAA+ ATPases plays a critical role in...

pectobacterium carotovorum is one of the most important bacterial pathogens of potato in the world.the expression of numerous genes including those involved in the production of virulence determinants in this bacterium are regulated by quorum sensing (qs). the signal molecules involved in qs in p. carotovorum belong to the group of n-acyl homoserine lactones (ahls). recently, several soil bacte...

conclusions phoma glomerata pg41 strain sharing natural substrate with toxigenic a. flavus secretes metabolites possessing a significant aflatoxin-degrading activity. the activity is associated mainly with a protein-enriched high-molecular-weight fraction of extracellular metabolites and appears to be of enzymatic origin. results the afb1-degrading activity of protein-enriched fractions, isolat...

in pakistan besides pesticide contamination from agricultural field, the manufacturing industriesare also contributing relatively high quantities of toxic pesticides into the environment. these pesticides maybe toxic, mutagenic or carginogenic and may be bioaccumulated or biomagnified by the biota. because ofpersistent nature of pesticides, its removal from environmental system become difficult...

Abstract Mycotoxins are generally found in food, feed, dairy products, and beverages, subsequently presenting serious human animal health problems. Not surprisingly, mycotoxin contamination has been a worldwide concern for many research studies. In this regard, biological, chemical, physical approaches were investigated to reduce and/or remove from food feed products. Biological detoxification ...