SYNOPSIS. In light-producing cells (photocytes) of the firefly light organ, mitochondria are clustered in the cell periphery, positioned between the tracheolar air supply and the oxygen-requiring bioluminescent reactants which are sequestered in more centrally-localized peroxisomes. This relative positioning suggests that mitochondria could control oxygen availability for the light reaction. We...

In light-producing cells (photocytes) of the firefly light organ, mitochondria are clustered in the cell periphery, positioned between the tracheolar air supply and the oxygen-requiring bioluminescent reactants which are sequestered in more centrally-localized peroxisomes. This relative positioning suggests that mitochondria could control oxygen availability for the light reaction. We hypothesi...

BACKGROUND Local ischemia before the development of recipient circulation may contribute to the highly variable long-term results of fat grafting. Remote ischemic preconditioning before adipose procurement augments the retention of fat grafts and limits subsequent liposclerosis. However, there is no literature examining what role remote ischemic preconditioning has on the fat graft recipient si...

Luminescent organisms have been observed throughout the ages. The cause of the luminescence wasn’t known until the advent of the microscope and modern science. In the oceans, sailors often remarked at the eerie glowing water generated by their vessels. Christopher Columbus, during his historic voyage across the Atlantic, noticed mysterious patches of luminescent light around the waters of his s...

Long-term treatment of HCT116.hCG.Luc orthotopic tumors: in vivo monitoring and survival To confirm our previous results in a second colon cancer cell line, thirty-one mice were intracecally implanted with HCT116.hCG.Luc. Two weeks later, mice were randomized according to bioluminescence signal and treatments with (1) control vehicle, (2) FOLFOX-like, (3) topotecan, (4) topotecan+pazopanib, (5)...

Bioluminescence (BL) relies on the enzymatic reaction between luciferase, a substrate conventionally named luciferin, and various cofactors. BL imaging has become widely used technique to interrogate gene expression cell fate, both in small large animal models of research. Recent developments include generation improved luciferase–luciferin systems for deeper more sensitive as well new caged lu...