The aim of the study is to construct a mathematical model kinetics multiple ethanol intake based on kinetic equation zero-order elimination with absorption and irrevocable first-order deficiency, as well its implementation in format computer program. Analytical modeling oral administration performed basis this equation. In course modeling, summation method was developed that allows perform desc...

Propionibacteria have mainly been found in dairy products and on the skin of humans animals. This genus classified as non-producing PHA but this study we show that Propionibacterium sp. can produce PHA. A member was isolated from soil. The isolate identified based its gram positive pleomorphic rods. Cells occur short chains, singly or pairs, V Y configurations. On mineral salt agar colonies are...

We have developed the conversion of glycerol into thermoplastic poly(3-hydroxypropionate) [poly(3HP)]. For this, the genes for glycerol dehydratase (dhaB1) of Clostridium butyricum, propionaldehyde dehydrogenase (pduP) of Salmonella enterica serovar Typhimurium LT2, and polyhydroxyalkanoate (PHA) synthase (phaC1) of Ralstonia eutropha were expressed in recombinant Escherichia coli. Poly(3HP) wa...

The aims of these experiments were treating tapioca processing waste water to produce polyhydroxyalkanoates (PHAs) and removal of chemical oxygen demand (COD). The experiments were conducted in a sequencing batch reactor with a volume of 6 L. The entire experimental condition was anaerobic-aerobic-anaerobic. Experiment 1, 2 and 3 had the first 2 h for feeding time while Experiment 4 had 3 h. Th...

Saline Mussels Cooking Wastewater was valorised to produce PHA with Mixed Microbial Cultures (MMC). Due the high protein content (1.8–5.7 g CODPROT/L), accumulating capacity below 10%, so several strategies were tested. In acidification unit, Na(HCO3) added, increasing conversion into Volatile Fatty Acids (VFA) from 10.3% 69.2% and subsequent accumulation 6.9 14.7%. enrichment incorporation of ...

The present study focuses on sustainable production of biodegradable polymers by <br /> Cupriavidus necator DSMZ 545 using glycerol as substrate. batch growth and biopolymer kinetics were established in a 7-L bioreactor, which resulted total biomass 8.88 g L–1 poly(3-hydroxybutyrate) (PHB) accumulation 6.76 L–1. kinetic independently acquired substrate inhibition data then used to develop...

Polyhydroxyalkanoates (PHA) and α-amylase (α-1,4 glucan-4-glucanohydrolase, E.C. 3.2.1.1) were co-produced by Bacillus sp. CFR-67 using unhydrolysed corn starch as a substrate. Bacterial growth and polymer production were enhanced with the supplementation of hydrolysates of wheat bran (WBH) or rice bran (RBH) individually or in combination (5-20 g L(-1), based on weight of soluble substrates-SS...

Cupriavidus necator DSM 545 is a well-known polyhydroxyalkanoates (PHAs) producer, but unable to grow on lactose. The aim of this study was to construct a recombinant strain of C. necator that can use lactose-containing waste material such as cheese whey, to produce PHAs. One of the intracellular PHA depolymerases (phaZ1) of C. necator was chosen to insert the lacZ, lacI and lacO genes of Esche...

Two low structured mathematical models for fed-batch production of polyhydroxybutyrate and poly[hydroxybutyrate-co-hydroxyvalerate] by Cupriavidus necator DSM 545 on renewable substrates (glycerol and fatty acid methyl esters-FAME) combined with glucose and valeric acid, were established. The models were used for development/optimization of feeding strategies of carbon and nitrogen sources conc...

Polyhydroxyalkanoates (PHAs) are a group of biopolymers used as an alternative to petroleum-based synthetic plastics. Their industrial application is not widely available due production cost constraints, mainly from raw materials for carbon sources. This research focuses on selecting Bacillus strain lipid-containing wastewater that can produce PHAs crude glycerol and investigating the optimum c...