نتایج جستجو برای: bioreactor
تعداد نتایج: 6462 فیلتر نتایج به سال:
OBJECTIVES Recombinant protein production processes in Escherichia coli are usually operated in fed-batch mode; therefore, the elaboration of a fed-batch cultivation protocol in microtiter plates that allows for screening under production like conditions is particularly appealing. RESULTS A highly reproducible fed-batch like microtiter plate cultivation protocol for E. coli in a micro-bioreac...
A major portion of the over expressed yeast mitochondrial aconitase, a large 82 kDa monomeric TCA cycle enzyme, in Escherichia coli led to the formation of inclusion bodies. Bacterial chaperonin GroEL mediated the correct folding of aconitase with the assistance of its co-chaperonin GroES in an ATP dependent manner. Till date the chaperonin assisted folding of aconitase was limited to the shake...
To achieve large-scale low-cost ex vitro acclimatization of Siberian ginseng plants, heart- and torpedo-shaped secondary somatic embryos (SEs) induced from germinated SEs on agar medium were collected and then inoculated to 10-l bubble column bioreactor, respectively. For plantlet conversion, inoculation of torpedo-shaped secondary SEs was more effective than heart-shaped SEs. TS2 (culture of t...
In the present study, two bench-scale anaerobic/ anoxic/ oxic submerged membrane bioreactors were used to study the effect of thermochemical sludge disintegration system on the excess sludge production. Among the two membrane bioreactors, one was named experimental membrane bioreactor and another one was named as control membrane bioreactor, where a part of the mixed liquor was treated with the...
Different types of stem cells have been investigated for applications in drug screening and toxicity testing. In order to provide sufficient numbers of cells for such in vitro applications a scale-up of stem cell culture is necessary. Bioreactors for dynamic three-dimensional (3D) culture of growing cells offer the option for culturing large amounts of stem cells at high densities in a closed s...
−Bacterial cellulose (BC) was produced by Acetobacter xylinum KJ1 in a modified airlift-type bubble column bioreactor, which had a low shear stress and high oxygen transfer rate (kLa). Saccharified food wastes (SFW) were used as the BC production medium due to its low cost. An aeration rate of 1.2 vvm (6 L/min) was tentatively determined as the optimal aeration condition in a 10 L spherical typ...
The replacement of petrochemicals with biobased chemicals requires efficient bioprocesses, biocatalysis, and product recovery. Biocatalysis (e.g., enzyme conversion and fermentation) offers an attractive alternative to chemical processing because biocatalysis utilize renewable feedstocks under benign reaction conditions. One class of chemical products that could be produced in large volumes by ...
INTRODUCTION Primary cells in vitro culture scale-up is a crucial issue in cell-based tissue and organ regeneration therapy. Reducing costs and space occupied by the cells cultured in vitro has been an important target. Cells cultured in vitro with the use of bioreactor with dextran microcarriers (Cytodex®) have potentially a chance to meet many of the cell therapy requirements. MATERIAL AND ...
Flow perfusion bioreactors have been shown to enhance fluid transport and improve cell viability throughout tissue-engineered bone constructs. Furthermore, osteoblasts have been shown to be stimulated by flow during bioreactor culture, although the optimum flow regime to promote an osteogenic response has yet to be found. One problem is that bone cells lose their ability to respond to stimulati...
Background Development of bioprocesses for animal cells has to deal with different bioreactor types and scales. Bioreactors might be intended for generation of cell inoculum and production, research, process development, validation or transfer purposes. During these activities, not only the difficulty of upand downscaling might lead to failure of consistency in cell growth, but also the use of ...
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