BACKGROUND Among disposable bioreactor systems, cylindrical orbitally shaken bioreactors show important advantages. They provide a well-defined hydrodynamic flow combined with excellent mixing and oxygen transfer for mammalian and plant cell cultivations. Since there is no known universal correlation between the volumetric mass transfer coefficient for oxygen kLa and relevant operating paramete...

There is an increasing interest in developing microfluidic bioreactors and organs-on-a-chip platforms combined with sensing capabilities for continual monitoring of cell-secreted biomarkers. Conventional approaches such as ELISA and mass spectroscopy cannot satisfy the needs of continual monitoring as they are labor-intensive and not easily integrable with low-volume bioreactors. This paper rep...

The accurate prediction of hepatotoxicity demands validated human in vitro models that can close the gap between preclinical animal studies and clinical trials. In this study we investigated the response of primary human liver cells to toxic drug exposure in a perfused microscale 3D liver bioreactor. The cellularized bioreactors were treated with 5, 10, or 30 mM acetaminophen (APAP) used as a r...

Tissue engineering is an exciting new area of medicine with rapid growth and expansion over the last decade. It has the potential to have a profound impact on the practice of medicine and influence the economic development in the industry of biotechnology. In almost every specialty of medicine, the ability to generate replacement cells and develop tissues will change the focus from artificial o...

A recent effort to improve malic acid production by Saccharomyces cerevisiae by means of metabolic engineering resulted in a strain that produced up to 59 g liter(-1) of malate at a yield of 0.42 mol (mol glucose)(-1) in calcium carbonate-buffered shake flask cultures. With shake flasks, process parameters that are important for scaling up this process cannot be controlled independently. In thi...

Bioreactors are noted for their dynamic behavior deviant from that of chemical reactors because of metabolic regulation. Consequently model-based control of bioreactors must rely on models that can accommodate regulatory behavior. Although the framework of kinetics, the hallmark of analysis of all chemical reaction systems (of which metabolism is but an illustrious member), would be a natural i...

The biodegradability ofN, N Octadecyldimethylamine, a long-chained aliphatic amine, was studied in a biotreatability system. The study serves as a precursor to the application ofcompost biofiltration to treat odorous waste gases containing long-chained alkyl amines (e.g., emitted by spray-painting operations). The biodegradability ofthe amine was determined by evaluating contaminant removal and...

Denitrification bioreactors for nitrate removal in agricultural drainage have recently gained attention for their low cost and effectiveness. While research continues to investigate the optimal sizing of these systems, little work has been done on the optimal design geometry (Length to width ratio and cross sectional shape). To address this lack, pilot-scale (1/10th scale) work performed in Iow...

UNLABELLED Two acid mine drainage (AMD) sites in the Appalachian bituminous coal basin were selected to enrich for Fe(II)-oxidizing microbes and measure rates of low-pH Fe(II) oxidation in chemostatic bioreactors. Microbial communities were enriched for 74 to 128 days in fed-batch mode, then switched to flowthrough mode (additional 52 to 138 d) to measure rates of Fe(II) oxidation as a function...

INTRODUCTION: To present, NMR-compatible bioreactors have been utilized as a platform to explore the metabolism of cells non-invasively. These systems have utilized standard 10–25mm coils to exploit filling factor and ease of construction [1-3]. For immortalized cell lines, densities on the order of 10 cells are achievable, requiring extensive cell culture, but this is nearly impossible to achi...