Many uses of emulsion droplets require precise control over droplet size and shape. Here we report a ‘shape-memorable’ micro-droplet formulation stabilized by polyethylene glycol (PEG)-modified protein -surfactant, the are stable against coalescence for months can maintain non-spherical shapes hours, depending on surface coverage PEGylated protein. Monodisperse with aspect ratios ranging from 1...

HIGHLIGHTS Recombinant E. coli can produce mono-rhamnolipid and di-rhamnolipid biosurfactants. Rhamnolipid congeners were identified using HRMS. Di-rhamnolipid has lower surface tension CMC than mono-rhamnolipid. production have been optimized to enhance rhamnolipid yield.