The objective of the study was to develop a high performance liquid chromatography (HPLC) method using ultra violet (UV) detection for the determination of flavoxate HCl in bulk and solid dosage forms by using ibuprofen as the internal standard. Eclipse C18 column (150 mm × 4.6 mm, 5 μm) was used as the stationary phase with a mixture of acetonitrile : 0.1% formic acid in water (75: 25 v/v) as ...

In this study, analytical and preparative HPLC systems were utilized to obtain isoflavones from Korean soybean. The mobile phases were the ternary system of water/acetonitrile/acetic acid. The optimum operating conditions were experimentally determined with the analytical column (C18, 0.46x25 cm 5 micron and the preparative column packed with 15 micron. The experimental variables were the gradi...

Objective of the present study is to develop analytical methods for simultaneous determination of two major biflavones, amentoflavone and heveaflavone, in Biyanling Tablets, thereby providing methodological reference for quality control of anticancer drug Biyanling Tablets. Samples are chromatographed on Diamonsil C18 (4.6 mm × 250 mm, 5 μm) column with a mobile phase of acetonitrile (B)–0.5% a...

The objective of this work was to develop and validate a rapid high performance liquid chromatography (HPLC) method for the quantitative analysis of fluorouracil (5-FU) in polymeric nanoparticles. Chromatographic analyses were performed on an RP C18 column with a mobile phase consisting of acetonitrile and water (10:90, v/v) at a flow rate of 1 mL/min. The 5-FU was detected and quantitated usin...

A validated HPLC method was developed to determine the doxorubicin concentration in a small volume of rat plasma (60 µL) with convenient fluorescence detection. Sample preparation includes a simple one-step liquid-liquid extraction using a minimum amount of organic solvent, with extraction recovery more than 95%. The analysis was accomplished using PerfectSil C18 column maintained at 35 °C and ...

Leflunomide (LLM) is subjected to forced degradation under conditions of hydrolysis, oxidation, dry heat, and photolysis as recommended by International Conference on Harmonization guideline Q1A(R2). In total, four degradation products (I-IV) were formed under different conditions. Products I, II and IV were formed in alkaline hydrolytic, acidic hydrolytic and alkaline photolytic conditions. LL...

An improved and reliable ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method has been developed and validated for the determination of lercanidipine in human plasma. Plasma samples with lercanidipine-d3 as an internal standard (IS) were prepared by solid phase extraction on Phenomenex Strata-X cartridges using 100 µL of human plasma. Chromatographic analysis was...

A stability-indicating ultra-performance liquid chromatography method was developed and validated for the simultaneous determination of a fixed dose combination of atorvastatin and ezetimibe in bulk drugs. The developed method was successfully applied to the simultaneous quantitative analysis of the combination drugs in tablet. The chromatographic separation was performed on a Kromasil Eternity...

In this work, a liquid chromatography stability-indicating method was developed and applied to study the hydrolytic behavior of simvastatin in different pH values and temperatures. The selected chromatographic conditions were a C18 column; acetonitrile-28 mM phosphate buffer solution, pH 4 (65 + 35) as the mobile phase; 251 degrees C column temperature; and flow rate 1 mL/min. The developed met...

In this study, purified peptides from shrimp waste hydrolysates (SWHs) were examined for their inhibitory effects against β–secretase. During consecutive purification using a Sephadex G–25 column chromatography and high performance liquid chromatography on a C18 column, a potent β–secretase inhibitory peptide Asp–Val–Leu–Phe– His (629 Da) was isolated and identified from SWH24 by Q–TOF MS/MS an...