The objective was to characterize transcriptome changes associated with elongation in bovine conceptuses during preimplantation stages. Nonlactating Holstein cows were euthanized 17 d after artificial insemination (AI) and the uterine horn ipsilateral CL flushed saline solution. Recovered classified as small (1.2 6.9 cm; n = 9), medium (10.5 16.0 or large (18.0 26.4 10). Total mRNA extracted su...

Microneutralization tests for detection of antibody in bovine serum to three bovine viruses are described. The Madin-Darby bovine kidney cell line was used with parainfluenza 3 virus (PI 3), whereas serially cultivated bovine embryonic kidney cells were used for infectious bovine rhinotracheitis virus and bovine virus diarrhea virus. Comparison of micro-hemagglutination-inhibition (HI) with mic...

Embryonic chick wing bud tissues secrete diffusible mitogens when cultured in vitro (Bell & McLachlan, 1985). These molecules may play an important role in limb development since media conditioned by morphogenetically active regions of the wing bud possess greater mitogenic activity than media conditioned by non-morphogenetic regions. These studies show that while the chick-derived growth facto...

Background: Addition of amino acids to the culture medium is beneficial for embryonic development in many species. Objective: The objective of this study was to investigate the effects of amino acids on the in vitro maturation and embryonic development of the bovine oocyte. Materials and Methods: Bovine ovaries were collected from a local abattoir and brought into laboratory. Cumulus-oocyte com...

PURPOSE A rapid means was sought to visualize and quantify the cross-sectional areas of fiber cells, the variations of cell area, and the regularity of packing in the equatorial plane of normal adult bovine and normal aged human lenses. METHODS Vibratome sections of bovine and human lenses were fixed, embedded in LR White resin, and sectioned for light microscopic observation. Image analysis ...

The study of large animal embryonic stem cells (ESCs) in vitro has implications for the understanding of lineage differentiation and transgenesis. The first step for ESC derivation is the attachment of the embryo to a substrate on which they can form outgrowths. However, the culture conditions for large animal embryo attachment and ESC derivation have not been studied extensively. Defining cult...