BACKGROUND The blaZ gene encoding penicillin resistance can be located either chromosomally or on plasmids. The aim of this study was to investigate the genetic relationships and to determine the location of the blaZ gene in S. aureus isolated in bovine mastitis in Finland and Sweden. METHODS Seventy-eight β-lactamase positive S. aureus isolates from bovine mastitis (34 from Finland and 44 fr...

Mastitis is the major cause of loss in dairy farming. Somatic cells are one of most important standards to detect this infection. This work applies to a blended segmentation method for counting them in bovine milk images. Firstly, it uses cloud model to threshold cell images; secondly, watershed is used to reconstruct distance image in order to obtain the initial segmentation result; finally, a...

The data here presented indicate that the inhibitory principle affecting the growth of streptococci in milk is not greatly utilized during the lag phase and that the abrupt termination of lag is not due to the utilization of the principle. They further indicate that the sudden beginning of growth cannot be ascribed to a resistant type of streptococcus present in the culture from the first, but ...

Pirlimycin is an analog of clindamycin that will be recommended for therapy of bovine mastitis. It has good activity against staphylococci and streptococci, the major pathogens for bovine mastitis. Five hundred and thirty bacterial isolates recovered from cows with mastitis were studied to confirm the spectrum of activity and to develop recommendations for susceptibility testing. Pirlimycin is ...

Bovine mastitis caused by Klebsiella pneumoniae is usually treated with antibiotics, thereby potentially increasing antimicrobial resistance. The objective of this study was to evaluate efficacy a bacteriophage, isolated from dairy farm wastewater, as treatment for murine model K. mastitis. A lytic bacteriophage CM8–1 isolated, morphological and biological characteristics were assessed transmis...

During bacterial infection of the bovine mammary gland, large numbers of leukocytes migrate into the udder, resulting in the establishment of a host response against the pathogen. Currently, the specific leukocyte populations mediating this immune response are not well defined. In the studies described here, we analyzed blood and milk from healthy cows and cows with naturally occurring mastitis...

Mastitis is inflammation of mammary gland and most devastating disease condition in terms of economic losses occurring throughout the world. The etiological agents may vary from place to place depending on climate; animal species and animal husbandry and include wide variety of gram positive and gram negative bacteria; and fungi. Conventional diagnostic tests viz. California Mastitis Test (CMT)...

Fibroblasts constitute the majority of the stromal cells within bovine mammary gland, yet the functional contributions of these cells to mastitis and fibrosis and the mechanism are poorly understood. In this study, we demonstrate that inflammation-associated fibroblasts (INFs) extracted from bovine mammary glands with clinical mastitis had different expression pattern regarding to several extra...

Staphylococcus aureus is a major pathogen that causes subclinical mastitis associated with huge economic losses to the dairy industry. A few vaccines for bovine mastitis are available, and they are expected to induce the production of S. aureus-specific antibodies that prevent bacterial adherence to host cells or promote opsonization by phagocytes. However, the efficacy of such vaccines are sti...