Though many diagnostic PCR methods have been described, few techniques have been developed to rapidly and efficiently screen transformed bacterial colonies for plasmid presence (1). Unfortunately, PCR screening of a large number of colonies is time consuming and requires substantial amounts of polymerase and primers. In this report, we describe a two-step large-scale bacterial colony screening ...

Neurons and neuroendocrine cells must retrieve plasma membrane excess and refill vesicle pools depleted by exocytosis. To perform these tasks cells can use different endocytosis/recycling mechanisms whose selection will impact on vesicle recycling time and secretion performance. We used FM1-43 to evaluate in the same experiment exocytosis, endocytosis, and recovery of releasable vesicles on mou...

Herein we report the synthesis, characterization and antioxidant activity of the Schiff base ligands (E)-4-bromo-2-((2-piperazine-1-yl)ethylimino)methylphenol and 2,2’-(1E,1E’)-cyclohexane1,2-diylbis(azan-1-yl-1-ylidene))bismethan-1-yl-1-ylidene)bis (4-bromophenol) and their complexes of copper (II) ion. Antioxidant activities of the copper complexes were studied by ferric reducing antioxidant ...

The Mongolian gerbil has been widely used as a global brain ischemia model because of its incomplete cerebral circle of Willis. However, the inter-individual anatomic variability of this vascular structure interferes with the reliability of the model. The aim of this work was to introduce modifications to the protocol of global brain ischemia experiments in Mongolian gerbils in an attempt to in...

Background: Juvenile idiopathic arthritis (JIA) comprises a heterogeneous group of chronic, childhood onset, autoimmune diseases with highly variable clinical presentations, outcomes and therapeutic responses. Although clinical evaluation has been a central and important method to pediatric rheumatologists of establishing likely diagnoses and outcomes, laboratory testing for pathogenic biomarke...

the objective of this study was to determine the efficacy of two procedures i.e. feeding and immersion followed by feeding, for masculinizing blue hap. two experiments (experiments i and ii) were conducted. in experiment i, feeding hormonal treatment was applied on post-yolk sac stage fry (10 days after fertilization at 27ºc). dosages of 30mg (group a) and 60mg (group b) of 17α-methyltestostero...