A rapid and efficient protocol was developed for inducing indirect organogenesis using leaf explants of Vitex negundo L. Explants were cultured on MS with different concentrations of 2,4-D and IAA in combination with BAP for callus induction. The frequency of callus induction increased with increasing concentration of IAA (0.3 mg/l) and BAP (0.3 mg/l) at optimal level. The shoot buds appeared e...

Prostate organogenesis is a complex process that is primarily mediated by the presence of androgens and subsequent mesenchyme-epithelial interactions. The investigation of prostate development is partly driven by its potential relevance to prostate cancer, in particular the apparent re-awakening of key developmental programs that occur during tumorigenesis. However, our current knowledge of the...

In Hydra, head regeneration and bud formation appear to be very similar processes. The fact that there are genes whose expression is specific for one of the two processes suggests that they do not have identical molecular bases. We analyzed the signal transduction pathways regulating bud development using inhibitors of protein kinase C, Src, PI3K and ERK. The four inhibitors reversibly blocked ...

Bud growth in yeast is guided by myosin-driven delivery of secretory vesicles from the mother cell to the bud. We find transport occurs along two sets of actin cables assembled by two formin isoforms. The Bnr1p formin assembles cables that radiate from the bud neck into the mother, providing a stable mother-bud axis. These cables also depend on septins at the neck and are required for efficient...

To determine whether the innervation of taste buds changes during postnatal development, the number of geniculate ganglion cells that innervated single fungiform taste buds were quantified in the tip- and midregions of the tongue of adult and developing rats. There was substantial variation in both the size of individual taste buds and number of geniculate ganglion cells that innervated them. I...

The classically defined induction of the liver from the endoderm, elicited by the cardiac mesoderm, has recently been discovered to involve signaling by fibroblast growth factors (FGFs). Multiple FGFs induce hepatic gene expression independent of an effect on growth. A subset of these FGFs cooperates with other factors to promote morphogenesis of the newly specified hepatocytes. Subsequent to t...

Photoperiod or daylength affects a diverse set of traits in plants, including flowering and tuberization in annuals, as well as growth cessation and bud set in perennials. During the last 10-15 years, great progress has been made in the understanding of molecular mechanisms controlling photoperiodic induction of flowering, in particular in the model species Arabidopsis thaliana. An obvious ques...

in this research, possibility of bulblet production from different cuttings including leaf, leaf-bud and leafy-stem of lilium oriental hybrid cv. ‘star gazer’ evaluated under different levels of indole-3-butyric acid (iba) with 0, 250, 500, 750 and 1000 mg per liter (ppm). the cuttings were taken from stems which grown from cold treated bulbs in early october. a factorial experiment was done in...

this study was performed to evaluate the efficacy of injection of essential oil of eugenia caryophyllata in the kid horn buds, as a new chemical technique for disbudding. five-day-old healthy goat kids from both sexes (n = 16) were divided randomly into 4 equal groups. in groups 1, 2 and 3, 0.2 ml of clove essence and in group 4 (control) 0.2 ml of normal saline was injected into the left horn ...

background: the purpose of dehorning in cattles is to decrease injuries and increase security in farms. besides breeding managements, different chemical and physical methods are used for dehorning of livestock, depending on their age, each of which has advantages and disadvantages. objectives: this study was performed to evaluate the effects of clove oil on disbudding new born calves following ...