BACKGROUND Reproductive disorders associated with chlamydial infection have been reported worldwide in cattle and there are indications of potential venereal transmission. METHODS Semen samples from 21 dairy bulls and cauda epididymidis tissue samples from 43 beef bulls were analysed for chlamydial agent by real-time polymerase chain reaction (PCR) including an internal amplification control ...

In this study, we determined species-specific variations by analyzing the mitochondrial 12S rRNA gene sequence variation (∼440 bp) in 17 newly obtained sequences and 90 published cattle, yak, buffalo, goat, and pig sequences, which represent 62 breeds and 17 geographic regions. Based on the defined species-specific variations, two endonucleases, Alu I and Bfa I, were selected for species authen...

The Malaysian water buffalo, Bubalus bubalis, has traditionally been an important livestock for the local people of Sarawak. Buffalo, other ruminants, and non-ruminants are essential industry as they supply largest source protein entire nation. Cattle provide production in at 0.2%, followed by including goats (0.1%), sheep (0.04%), buffalo (0.03%). Water or 'kerbau sawah' it is locally known, o...

average age distribution and generation interval were investigated in four different selection pathways. i.e., sire of future sires (ss), sire of future dams (sd), dam of future sires (ds) and dam of future dams (dd). young bulls pathway (yb) is also considered as a path that affects the generation interval in sd pathway when using progeny testing program. data were collected from animal breedi...

*Corresponding Author Rajesh. K. Patel [email protected] The present study investigated the occurrence of an autosomal recessive genetic disease, Bovine Citrullinaemia caused by mutation in Argininosuccinate Synthase (ASS) gene, in Indian Holstein cattle. The Polymerase chain reactionRestriction fragment length polymorphism (PCR-RFLP) analysis was performed on a group of 120 Holstein bulls to...

In this study we evaluated the effects of semen extender supplementation with different concentrations of glutathione (GSH) on buffalo (Bubalus bubalis) bull sperm motility, plasma membrane integrity, viability and DNA integrity as well as in vivo fertility. Semen from three Nili-Ravi buffalo bulls was collected, and qualified semen ejaculates (n=18) were split into five aliquots for dilution (...

AIM A comparative study was conducted on crossbred cattle and buffaloes to investigate the effect of feeding high and low roughage total mixed ration (TMR) diets on rumen metabolites and enzymatic profiles. MATERIALS AND METHODS Three rumen-fistulated crossbred cattle and buffalo were randomly assigned as per 3×3 switch over design for 21-days. Three TMR diets consisting of concentrate mixtur...

This study was carried out in 1001 cattle that were slaughtered in 3 abattoirs in the Afyonkarahisar district of western Turkey. Abattoirs were visited twice a week and internal organs of the cattle were examined for cystic echinococcosis (CE). The number of organs discarded because of CE was recorded. CE was found in 295 (29.47%) out of 1001 cattle studied. CE was found in 113 (44.31%) out of ...

The aim of the present study was to assess the effect of addition of Royal jelly in presence of heparin on buffalo (Bubalus Bubalis) sperm motility, acrosome reaction and in vitro fertilization (IVF) of buffalo oocytes. Frozen buffalo spermatozoa from five bulls were thawed and motile fraction was obtained by swim up technique. The spermatozoa were washed, treated with100 μg/ml heparin, and the...

diacylglycerol acyltransferase1 (dgat1) is a key enzyme in synthesis of triglycerides. a transition mutation resulting substitution of guanine by adenine in dgat1 gene and substitution of lysine by alanine in diacylglycerol-acyltransferase enzyme in cattle has a major effect on milk yield and milk composition traits. in this research, the polymorphism of the region spanning exon 3 to exon 17 of...