نتایج جستجو برای: bulked segregant analysis
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In crops such as Brassica rapa, the agronomic trait plant height affects leaf light absorption rate, benefits substance accumulation, and increases production by increasing biological yield of population. The mechanism was adequately studied in napus Arabidopsis, while rapa had been rarely reported. Herein, we a B. dwarf mutant Brcd1YS. Compared with its wild-type, Yellow sarson, Brcd1YS showed...
Introduction Multiple nodes and dwarf mutants in barley are a valuable resource for identifying genes that control shoot branching, vegetative growth development. Methods In this study, physiological, microscopic genetic analysis were conducted to characterize fine-map the underling gene of mutant with Stem Nodes Spikes Dwarf ( msnsd ), which was selected from EMS- 60 Co-treated cv . Edamai 934...
Peel color is one of the most important appearance qualities eggplant. The main pigment in peel green-fruited eggplant chlorophyll, while white-fruited a mutant chlorophyll biosynthesis. A dominant gene Gv controls biosynthesis peel, but none its genes have been mapped. In this study, white-peel inbred line 19141, green-peel 19143, and their F2 progeny with 3:1 segregation ratio plants to plant...
Fruit firmness is an important target of melon breeding, as it associated with shelf life and economic value; however, the precise mechanism that determines fruit during ripening remains elusive. In present study, one hundred forty-four F2 plants F2–3 families derived from high-firmness line M2–10 low-firmness ZT091 were used to identify major quantitative trait loci by specific-locus amplified...
Chinese cabbage (Brassica rapa L.) is one of the most important and highly nutritious vegetables in China belonging to Brassicaceae family. Flowering or bolting critical developmental stages flowering plants. For spring-sown cabbage, late-bolting desirable over early-bolting according consumer preferences. We determined inheritance pattern trait using F1 F2 generated from a cross between ‘SY200...
Wheat cultivar VL Gehun 892 has shown a high level of resistance against Australian Puccinia striiformis f. sp. tritici (Pst) pathotypes. In this study, it was crossed with Westonia, susceptible wheat cultivar, and digenic segregation observed in the derived population Pst pathotype 134 E16A+Yr17+Yr27+. Single-gene recombinant inbred line (RIL) populations were developed from F3 families (VL 89...
In order to map the autosomal dwarf (adw) locus in the chicken, 11 segregating families were created. Initially five of these families were used for a linkage experiment in which the genome was scanned with microsatellites using a technique called bulked segregant analysis. Subsequently animals from 11 families were typed individually for microsatellites that appeared to be linked. We were able...
With the advance of next-generation sequencing (NGS) technologies, increasingly ambitious applications are becoming feasible. A particularly powerful one is the sequencing of polymorphic, pooled samples. The pool can be naturally occurring, as in the case of multiple pathogen strains in a blood sample, multiple types of cells in a cancerous tissue sample, or multiple isoforms of mRNA in a cell....
Rice (Oryza sativa L. cv Oochikara) is a typical silicon-accumulating plant, but the mechanism responsible for the high silicon uptake by the roots is poorly understood. We characterized the silicon uptake system in rice roots by using a low-silicon rice mutant (lsi1) and wild-type rice. A kinetic study showed that the concentration of silicon in the root symplastic solution increased with incr...
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